Files and links (1)
CC BY-NC-ND V4.0, Open Access
Journal article
Ecological incidence, genetic diversity, multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms
Poultry science, Vol.103(2), 103320
2023
Abstract
Salmonella is a significant foodborne pathogen that has a significant impact on public health, and different strains of multidrug resistance (MDR) have been identified in this genus. This study used a combination of phenotypic and genotypic approaches to identify distinct Salmonella species collected from poultry broiler and layer farms, and antibiotic sensitivity testing was performed on these species. A total of 56 Salmonella isolates were serotyped, and phenotypic antibiotic resistance was determined for each strain. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method was also used to provide a genotypic description, from which a dendrogram was constructed and the most likely phylogenetic relationships were applied. Salmonella isolates were detected in 20 (17%) out of 117 samples collected from small-scale broiler flocks. Salmonella isolates were classified as MDR strains after showing tolerance to 4 antibiotics, but no resistance to cloxacillin, streptomycin, vancomycin, or netilmicin was observed. From a genotypic perspective, these strains lack dfrD, parC, and blasfo-1 resistant genes, while harboring blactx-M, blaDHA-L, qnrA, qnrB, qnrS, gyrA, ermA, ermB, ermC, ermTR, mefA, msrA, tet A, tet B, tet L, tet M resistance genes. The genotyping results obtained with ERIC-PCR allowed isolates to be classified based on the source of recovery. It was determined that Salmonella strains displayed MDR, and many genes associated with them. Additionally, the ERIC-PCR procedure aided in the generation of clusters with biological significance. Extensive research on Salmonella serotypes is warranted, along with the implementation of long-term surveillance programs to monitor MDR Salmonella serotypes in avian-derived foods.
Details
- Title
- Ecological incidence, genetic diversity, multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms
- Authors/Creators
- Mona M. Elsayed - Mansoura UniversityYara F.H. El-Basrey - Zagazig UniversityAmira Hussein El-Baz - Mansoura UniversityHeba A. Dowidar - Higher Institute of EngineeringAshwag Shami - Princess Nourah bint Abdulrahman UniversityFatimah A. Al-Saeed - King Khalid UniversityAwad Alsamghan - King Khalid UniversityHeba M. Salem - Cairo UniversityWafaa Ahmed Alhazmi - King Abdulaziz UniversityKhaled A. El-TarabilyMariam H.E. khedr - Zagazig University
- Publication Details
- Poultry science, Vol.103(2), 103320
- Publisher
- Elsevier Inc. on behalf of Poultry Science Association Inc.
- Number of pages
- 13
- Grant note
- Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia: PNURSP2023R31 King Khalid University: R.G.P2 161-44 Abu Dhabi Award for Research Excellence, Department of Education and Knowledge: 21S105
The authors gratefully acknowledge Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2023R31) , Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. Authors are grateful to the deanship of King Khalid University for supporting this work under the grant number (R.G.P2 161-44) . This research was funded by the Abu Dhabi Award for Research Excellence, Department of Education and Knowledge (Grant #: 21S105) to K. A. El-Tarabily.
- Identifiers
- 991005625269507891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Centre for Terrestrial Ecosystem Science and Sustainability
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
1 File views/ downloads
18 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.376 Salmonella and Campylobacter
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- ESI research areas
- Plant & Animal Science