Journal article
Ectopic Expression of Transcription Factor NF-E2 Alters the Phenotype of Erythroid and Monoblastoid Cells
The Journal of biological chemistry, Vol.275(33), pp.25292-25298
2000
PMID: 10842186
Abstract
In this study, regulation of transcription factor NF-E2 was examined in differentiating erythroid and myeloid cells, and the impact of raising NF-E2 concentrations within these cell types was assessed. NF-E2 was expressed in the J2E erythroid cell line, but the levels increased only marginally during erythropoietin-induced differentiation. In contrast, rare myeloid variants of J2E cells did not express NF-E2. Although NF-E2 was present in M1 monoblastoid cells, it was undetectable as these cells matured into macrophages. Compared with erythroid cells, transcription of the NF-E2 gene was reduced, and the half-life of the mRNA was significantly shorter in monocytoid cells. Ectopic expression of NF-E2 had a profound impact upon the J2E cells; morphologically mature erythroid cells spontaneously emerged in culture, but the cells failed to synthesize hemoglobin, even in the presence of erythropoietin. Although proliferation and viability increased in the NF-E2-transfected J2E cells, their responsiveness to erythropoietin was severely diminished. Strikingly, increasing the expression of NF-E2 in M1 cells produced sublines that contained erythroid or immature megakaryocytic cells. Finally, overexpression of NF-E2 in primary hemopoietic progenitors from fetal liver increased erythroid colony formation in the absence of erythropoietin. These data demonstrate that elevated NF-E2 (i) had a dominant effect on the phenotype and maturation of J2E erythroid cells, (ii) was able to reprogram the M1 monocytoid line, and (iii) promoted the development of erythroid colonies by normal progenitors.
Details
- Title
- Ectopic Expression of Transcription Factor NF-E2 Alters the Phenotype of Erythroid and Monoblastoid Cells
- Authors/Creators
- Melissa S. Sayer - Royal Perth HospitalPeta A. Tilbrook - Royal Perth HospitalAngelo Spadaccini - Royal Perth HospitalEvan Ingley - Royal Perth HospitalMohinder K. Sarna - Royal Perth HospitalJames H. Williams - Royal Perth HospitalNancy C. Andrews - Howard Hughes Medical InstituteS. Peter Klinken - Royal Perth Hospital
- Publication Details
- The Journal of biological chemistry, Vol.275(33), pp.25292-25298
- Publisher
- Elsevier Inc.
- Identifiers
- 991005614922807891
- Copyright
- © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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