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Journal article
Effect of Deferoxamine on Post-Transfusion Iron, Inflammation, and In Vitro Microbial Growth in a Canine Hemorrhagic Shock Model: A Randomized Controlled Blinded Pilot Study
Veterinary sciences, Vol.10(2), Art. 121
2023
PMID: 36851425
Abstract
Red blood cell (RBC) transfusion is associated with recipient inflammation and infection, which may be triggered by excessive circulating iron. Iron chelation following transfusion may reduce these risks. The aim of this study was to evaluate the effect of deferoxamine on circulating iron and inflammation biomarkers over time and in vitro growth of Escherichia coli (E. coli) following RBC transfusion in dogs with atraumatic hemorrhage. Anesthetized dogs were subject to atraumatic hemorrhage and transfusion of RBCs, then randomized to receive either deferoxamine or saline placebo of equivalent volume (n = 10 per group) in a blinded fashion. Blood was sampled before hemorrhage and then 2, 4, and 6 h later. Following hemorrhage and RBC transfusion, free iron increased in all dogs over time (both p < 0.001). Inflammation biomarkers interleukin-6 (IL6), CXC motif chemokine-8 (CXCL8), interleukin-10 (IL10), and keratinocyte-derived chemokine (KC) increased in all dogs over time (all p < 0.001). Logarithmic growth of E. coli clones within blood collected 6 h post-transfusion was not different between groups. Only total iron-binding capacity was different between groups over time, being significantly increased in the deferoxamine group at 2 and 4 h post-transfusion (both p < 0.001). In summary, while free iron and inflammation biomarkers increased post-RBC transfusion, deferoxamine administration did not impact circulating free iron, inflammation biomarkers, or in vitro growth of E. coli when compared with placebo.
Details
- Title
- Effect of Deferoxamine on Post-Transfusion Iron, Inflammation, and In Vitro Microbial Growth in a Canine Hemorrhagic Shock Model: A Randomized Controlled Blinded Pilot Study
- Authors/Creators
- Melissa A. Claus - School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia Perth Veterinary Specialists, Osborne Park, WA 6017, Australia Small Animal Specialist Hospital, Tuggerah, NSW 2259, Australia Forensic Sciences Laboratory, ChemCentre, Resources and Chemistry Precinct, Bentley, WA 6102, Australia School of Science, Edith Cowan University, Joondalup, WA 6027, Australia Intensive Care Unit, Rockingham General Hospital, Cooloongup, WA 6168, Australia Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands Intensive Care Unit, Fiona Stanley Hospital, Murdoch, WA 6150, Australia School of Medicine, University of Western Australia, Crawley, WA 6009, AustraliaLisa Smart - School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia Perth Veterinary Specialists, Osborne Park, WA 6017, Australia Small Animal Specialist Hospital, Tuggerah, NSW 2259, Australia Forensic Sciences Laboratory, ChemCentre, Resources and Chemistry Precinct, Bentley, WA 6102, Australia School of Science, Edith Cowan University, Joondalup, WA 6027, Australia Intensive Care Unit, Rockingham General Hospital, Cooloongup, WA 6168, Australia Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands Intensive Care Unit, Fiona Stanley Hospital, Murdoch, WA 6150, Australia School of Medicine, University of Western Australia, Crawley, WA 6009, AustraliaAnthea L. Raisis - School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia Perth Veterinary Specialists, Osborne Park, WA 6017, Australia Small Animal Specialist Hospital, Tuggerah, NSW 2259, Australia Forensic Sciences Laboratory, ChemCentre, Resources and Chemistry Precinct, Bentley, WA 6102, Australia School of Science, Edith Cowan University, Joondalup, WA 6027, Australia Intensive Care Unit, Rockingham General Hospital, Cooloongup, WA 6168, Australia Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands Intensive Care Unit, Fiona Stanley Hospital, Murdoch, WA 6150, Australia School of Medicine, University of Western Australia, Crawley, WA 6009, AustraliaClaire R. Sharp - Murdoch University, Centre for Terrestrial Ecosystem Science and SustainabilitySam Abraham - Murdoch University, Centre for Biosecurity and One HealthJoel P. A. Gummer - ChemCentreMartin K. Mead - Murdoch UniversityDamian L. Bradley - School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia Perth Veterinary Specialists, Osborne Park, WA 6017, Australia Small Animal Specialist Hospital, Tuggerah, NSW 2259, Australia Forensic Sciences Laboratory, ChemCentre, Resources and Chemistry Precinct, Bentley, WA 6102, Australia School of Science, Edith Cowan University, Joondalup, WA 6027, Australia Intensive Care Unit, Rockingham General Hospital, Cooloongup, WA 6168, Australia Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands Intensive Care Unit, Fiona Stanley Hospital, Murdoch, WA 6150, Australia School of Medicine, University of Western Australia, Crawley, WA 6009, AustraliaRachel Van Swelm - Radboud University NijmegenErwin T. G. Wiegerinck - Radboud University NijmegenEdward Litton - Fiona Stanley Hospital
- Publication Details
- Veterinary sciences, Vol.10(2), Art. 121
- Publisher
- MDPI
- Grant note
- AC019 / American College of Veterinary Emergency and Critical Care Intensive Care Foundation of Australia and New Zealand (Trainee Grant) National Collaborative Research Infrastructure Strategy Murdoch University Veterinary Life Sciences Small Grant Murdoch University Research Training Program Scholarship
- Identifiers
- 991005565369407891
- Copyright
- © 2023 by the authors.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.573 Iron Metabolism
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