Journal article
Effect of copper, zinc and cadmium on the promoter of selenoprotein W in glial and myoblast cells
Journal of Inorganic Biochemistry, Vol.91(2), pp.356-362
2002
Abstract
Rat selenoprotein W (SeW) promoter activity was investigated using different concentrations of cadmium, copper, and zinc. Two fragments (404 and 1265 bp) of the SeW promoter, containing a single metal response element (MRE), were ligated into the multiple cloning site of a pGL3-Basic reporter plasmid. The constructs were transfected into cultured C6 (rat glial) and L8 (myoblast) cells and promoter activity measured by means of luciferase reporter gene fused to the SeW promoter fragments in the reporter plasmid. With post-transfection exposure of these cell lines to these metals, copper and zinc, but not cadmium, significantly increased promoter activity of the unmutated 1265 bp (not 404 bp) construct (p<0.05) only in the C6 cells. Mutation of the MRE sequence abolished promoter response to metal exposure but did not eliminate promoter activity. The results suggest that SeW expression in glial cells can be increased on exposure to copper and zinc and that this response is dependent on the MRE sequence present in the SeW promoter.
Details
- Title
- Effect of copper, zinc and cadmium on the promoter of selenoprotein W in glial and myoblast cells
- Authors/Creators
- A. Amantana (Author/Creator) - Oregon State UniversityW.R. Vorachek (Author/Creator) - Oregon State UniversityJ.A. Butler (Author/Creator) - Oregon State UniversityN.D. Costa (Author/Creator) - Murdoch UniversityP.D. Whanger (Author/Creator) - Oregon State University
- Publication Details
- Journal of Inorganic Biochemistry, Vol.91(2), pp.356-362
- Publisher
- Elsevier BV
- Identifiers
- 991005543243707891
- Copyright
- © 2002 Elsevier Science Inc.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
51 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.91 Contamination & Phytoremediation
- 3.91.920 Selenium
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Inorganic & Nuclear
- ESI research areas
- Biology & Biochemistry