Journal article
Effect of dung burial by the dung beetle Bubas bison on numbers and viability of Cryptosporidium oocysts in cattle dung
Experimental Parasitology, Vol.129(1), pp.1-4
2011
Abstract
Cryptosporidium oocysts were inoculated into fresh dung (∼1.2×104 oocysts per gram wet weight) and fed to dung beetles to assess the effect of dung burial by the dung beetle Bubas bison on the distribution of the oocysts in small cores of soil in the laboratory. The experiment consisted of five replicates of each of two treatments; controls (dung but no dung beetles) and the experimental treatment (inoculated dung and seven pairs of dung beetles). After 5 days, when approximately 90% of the dung was buried, the surface and buried dung was recovered and subsampled. The oocysts in the subsamples were recovered and enumerated using qPCR. Oocyst viability was evaluated using an assay based on the exclusion or inclusion of two fluorogenic vital dyes, 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI). Results revealed that overall 13.7% of oocysts remained on the surface and 86.3% of oocysts were buried. The viability of oocysts in buried dung was only 10% compared to oocysts the surface dung (58%). Therefore, widespread dung burial by B. bison during the winter months could substantially reduce the numbers of Cryptosporidium oocysts available to be washed into waterways following winter rains.
Details
- Title
- Effect of dung burial by the dung beetle Bubas bison on numbers and viability of Cryptosporidium oocysts in cattle dung
- Authors/Creators
- U. Ryan (Author/Creator) - Murdoch UniversityR. Yang (Author/Creator) - Murdoch UniversityC. Gordon (Author/Creator) - Water Corporation of Western Australia (Australia)B. Doube (Author/Creator) - Dung Beetle Solutions Australia, 37 Cave Avenue, Bridgewater, South Australia 5155, Australia
- Publication Details
- Experimental Parasitology, Vol.129(1), pp.1-4
- Publisher
- Academic Press
- Identifiers
- 991005542371707891
- Copyright
- © 2011 Elsevier Inc.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology