Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.)

Pooja Sihag; Upendra Kumar; Vijeta Sagwal; Prexha Kapoor; Yogita Singh; Sheetal Mehla; Priyanka Balyan; Reazul Rouf Mir; Rajeev K. K. Varshney; Krishna Pal Singh; Om Parkash Dhankher

doi:10.1002/tpg2.20307

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Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.)

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Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.)

Pooja Sihag, Upendra Kumar, Vijeta Sagwal, Prexha Kapoor, Yogita Singh, Sheetal Mehla, Priyanka Balyan, Reazul Rouf Mir, Rajeev K. K. Varshney, Krishna Pal Singh, …

The plant genome, Vol.17(1), e20307

2023

DOI: https://doi.org/10.1002/tpg2.20307

PMID: 36751876

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Abstract

The grain-filling stage in Triticum aestivum (wheat) is highly vulnerable to increasing temperature as terminal heat stress diminishes grain quality and yield. To examine the mechanism of terminal heat tolerance, we performed the biochemical and gene expression analyses using two heat-tolerant (WH730 and WH1218) and two heat-sensitive (WH711 and WH157) wheat genotypes. We observed a significant increase in total soluble sugar (25%-47%), proline (7%-15%), and glycine betaine (GB) (22%-34%) contents in flag leaf, whereas a decrease in grain-filling duration, 1000-kernel weight (8%-25%), and grain yield per plant (11%-23%) was observed under the late-sown compared to the timely sown. The maximum content of osmolytes, including total soluble sugar, proline, and GB, was observed in heat-tolerant genotypes compared to heat-sensitive genotypes. The expression of 10 heat-responsive genes associated with heat shock proteins (sHsp-1, Hsp17, and HsfA4), flavonoid biosynthesis (F3 '-1 and PAL), beta-glucan synthesis (CslF6 and CslH), and xyloglucan metabolism (XTH1, XTH2, and XTH5) was studied in flag leaf exposed to different heat treatments (34, 36, 38, and 40 degrees C) at 15 days after anthesis by quantitative real-time polymerase chain reaction. A significant increase in the relative fold expression of these genes with increasing temperature indicated their involvement in providing heat-stress tolerance. The high differential expression of most of the genes in heat-tolerant genotype "WH730" followed by "WH1218" indicates the high adaptability of these genotypes to heat stress compared to heat-sensitive wheat genotypes. Based on the previous results, "WH730" performed better in terms of maximum osmolyte accumulation, grain yield, and gene expression under heat stress.

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Title: Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.)
Authors/Creators: Pooja Sihag - Chaudhary Charan Singh Haryana Agricultural University
Upendra Kumar - Chaudhary Charan Singh Haryana Agricultural University
Vijeta Sagwal - Chaudhary Charan Singh Haryana Agricultural University
Prexha Kapoor - Chaudhary Charan Singh Haryana Agricultural University
Yogita Singh - Chaudhary Charan Singh Haryana Agricultural University
Sheetal Mehla - Chaudhary Charan Singh Haryana Agricultural University
Priyanka Balyan - Chaudhary Charan Singh University
Reazul Rouf Mir - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
Rajeev K. K. Varshney - Murdoch University, Food Futures Institute
Krishna Pal Singh - Govind Ballabh Pant University of Agriculture and Technology
Om Parkash Dhankher - University of Massachusetts Amherst
Publication Details: The plant genome, Vol.17(1), e20307
Publisher: Wiley Periodicals LLC on behalf of Crop Science Society of America.
Number of pages: 14
Grant note: SPARC/2018-2019/P854/SL / Scheme for Promotion of Academic and Research Collaboration
Identifiers: 991005602258107891
Copyright: © 2023 CCS Haryana Agricultural University.
Murdoch Affiliation: Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.49 Plant Stress Responses
Web Of Science research areas: Genetics & Heredity; Plant Sciences
ESI research areas: Plant & Animal Science