Journal article
Effect of using frozen–thawed bovine semen contaminated with lumpy skin disease virus on in vitro embryo production
Transboundary and Emerging Diseases, Vol.66(4), pp.1539-1547
2019
Abstract
Lumpy skin disease (LSD) is an important transboundary animal disease of cattle with significant economic impact because of the implications for international trade in live animals and animal products. LSD is caused by a Capripoxvirus, LSD virus (LSDV), and results in extensive hide and udder damage, fever and pneumonia. LSDV can be shed in semen of infected bulls for prolonged periods and transmitted venereally to cows at high doses. This study examined the effects of LSDV in frozen‐thawed semen on in vitro embryo production parameters, including viral status of media and resulting embryos. Bovine oocytes were harvested from abattoir‐collected ovaries and split into three experimental groups. After maturation, the oocytes were fertilized in vitro with frozen‐thawed semen spiked with a high (HD) or a lower (LD) dose of LSDV, or with LSDV‐free semen (control). Following day 7 and day 8 blastocyst evaluation, PCR and virus isolation were performed on all embryonic structures. After completing sufficient replicates to reach 1,000 inseminated oocytes, further in vitro fertilization (IVF) runs were performed to provide material for electron microscopy (EM) and embryo washing procedures. Overall, in vitro embryo yield was significantly reduced by the presence of LSDV in frozen‐thawed semen, irrespective of viral dose. When semen with a lower viral dose was used, significantly lower oocyte cleavage rates were observed. LSDV could be detected in fertilization media and all embryo structures, when higher doses of LSDV were present in the frozen‐thawed semen used for IVF. Electron microscopy demonstrated LSDV virions inside blastocysts. Following the International Embryo Transfer Society washing procedure resulted in embryos free of viral DNA; however, this may be attributable to a sampling dilution effect and should be interpreted with caution. Further research is required to better quantify the risk of LSDV transmission via assisted reproductive procedures.
Details
- Title
- Effect of using frozen–thawed bovine semen contaminated with lumpy skin disease virus on in vitro embryo production
- Authors/Creators
- C.H. Annandale (Author/Creator) - Onderstepoort Veterinary Academic HospitalM.P. Smuts (Author/Creator) - Onderstepoort Veterinary Academic HospitalK. Ebersohn (Author/Creator) - University of PretoriaL. du Plessis (Author/Creator) - Onderstepoort Veterinary Academic HospitalP.N. Thompson (Author/Creator) - Onderstepoort Veterinary Academic HospitalE.H. Venter (Author/Creator) - James Cook UniversityT.A.E. Stout (Author/Creator) - Utrecht University
- Publication Details
- Transboundary and Emerging Diseases, Vol.66(4), pp.1539-1547
- Publisher
- Blackwell-Wiss.-Verl
- Identifiers
- 991005540633907891
- Copyright
- © 2019 Blackwell Verlag GmbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
63 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.1662 Poxvirus Immunology
- Web Of Science research areas
- Infectious Diseases
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science