Journal article
Effects of antiphospholipid antibodies on in vitro platelet aggregation
Clinical and Applied Thrombosis/Hemostasis, Vol.18(1), pp.59-65
2012
Abstract
Antiphospholipid antibodies contribute to the development of thrombosis, although precise mechanisms remain to be elucidated. We determined the effects of affinity-purified anti-beta2-glycoprotein 1 (anti-β2GP1) and anti-prothrombin (anti-PT) antibodies on in vitro platelet aggregation. Adenosine diphosphate (ADP) and collagen-induced platelet aggregation were performed using platelet-rich plasma ([PRP] 250 × 109/L). Antiphospholipid antibodies (1.25-10 μg/mL) were preincubated with PRP for 10 minutes at 37°C prior to the addition of agonist. Anti-β2GP1 antibodies significantly reduced platelet aggregation (percentage area under the curve; %AUC) in a concentration-dependent manner using both 5 μmol/L (P < .001) and 2.5 μmol/L (P = .038) ADP but did not significantly affect the rate of aggregation. Anti-PT antibodies significantly enhanced 5 µg/mL collagen-induced platelet aggregation (%AUC; P = .034) but did not affect ADP-induced platelet aggregation. These results suggest (1) interactions and effects of antiphospholipid antibodies on platelets are agonist and concentration dependent and (2) anti-β2GP1 antibodies may inhibit dense granule release and/or inhibition of the arachidonic acid pathway.
Details
- Title
- Effects of antiphospholipid antibodies on in vitro platelet aggregation
- Authors/Creators
- A.A. Palatinus (Author/Creator) - University of TasmaniaK.D.K. Ahuja (Author/Creator) - University of TasmaniaM.J. Adams (Author/Creator) - University of Tasmania
- Publication Details
- Clinical and Applied Thrombosis/Hemostasis, Vol.18(1), pp.59-65
- Publisher
- SAGE Publications
- Identifiers
- 991005542129507891
- Copyright
- © The Author(s) 2012
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.75 Blood Clotting
- 1.75.326 Antiphospholipid Syndrome
- Web Of Science research areas
- Hematology
- Peripheral Vascular Disease
- ESI research areas
- Clinical Medicine