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CC BY V4.0, Open Access
Journal article
Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of Cannabis sativa
Plant-environment interactions (Hoboken, N.J. : 2018), Vol.4(3), pp.115-133
2023
PMID: 37362423
Abstract
Fungal pathogens pose a major threat to Cannabis sativa production, requiring safe and effective management procedures to control disease. Chitin and chitosan are natural molecules that elicit plant defense responses. Investigation of their effects on C. sativa will advance understanding of plant responses towards elicitors and provide a potential pathway to enhance plant resistance against diseases. Plants were grown in the in vitro Root‐TRAPR system and treated with colloidal chitin and chitosan. Plant morphology was monitored, then plant tissues and exudates were collected for enzymatic activity assays, phytohormone quantification, qPCR analysis and proteomics profiling. Chitosan treatments showed increased total chitinase activity and expression of pathogenesis‐related (PR) genes by 3–5 times in the root tissues. In the exudates, total peroxidase and chitinase activities and levels of defense proteins such as PR protein 1 and endochitinase 2 were increased. Shoot development was unaffected, but root development was inhibited after chitosan exposure. In contrast, chitin treatments had no significant impact on any defense parameters, including enzymatic activities, hormone quantities, gene expression levels and root secreted proteins. These results indicate that colloidal chitosan, significantly enhancing defense responses in C. sativa root system, could be used as a potential elicitor, particularly in hydroponic scenarios to manage crop diseases.
Chitosan elicits defense responses of Cannabis sativa root systems, but chitin has no effect in this regard. Defense enzymes and proteins were found to be increasingly produced in the tissues and secreted into exudate upon chitosan treatment. These results highlight the potential of chitosan to be alternatively used for managing fungal infection in agriculture, thereby minimizing the usage of chemicals.
Details
- Title
- Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of Cannabis sativa
- Authors/Creators
- Pipob Suwanchaikasem - The University of MelbourneShuai Nie - The University of MelbourneAlexander Idnurm - The University of MelbourneJamie Selby-Pham - The University of MelbourneRobert Walker - The University of MelbourneBerin A. Boughton - Murdoch University, Centre for Computational and Systems Medicine
- Publication Details
- Plant-environment interactions (Hoboken, N.J. : 2018), Vol.4(3), pp.115-133
- Publisher
- New Phytologist Foundation and John Wiley & Sons Ltd.
- Number of pages
- 19
- Grant note
- Nutrifield Pty Ltd SEED19 grant, School of BioSciences, University of Melbourne
- Identifiers
- 991005590369007891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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