Effects of dietary fish oil and vitamin E supplementation on canine lymphocyte proliferation evaluated using a flow cytometric technique

Casey J. LeBlanc; Marilyn A. Dietrich; David W. Horohov; John E. Bauer; Giselle Hosgood; Glenna E. Mauldin

doi:10.1016/j.vetimm.2007.05.014

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Effects of dietary fish oil and vitamin E supplementation on canine lymphocyte proliferation evaluated using a flow cytometric technique

Journal article

Peer reviewed

Effects of dietary fish oil and vitamin E supplementation on canine lymphocyte proliferation evaluated using a flow cytometric technique

Casey J. LeBlanc, Marilyn A. Dietrich, David W. Horohov, John E. Bauer, Giselle Hosgood and Glenna E. Mauldin

Veterinary immunology and immunopathology, Vol.119(3), pp.180-188

2007

DOI: https://doi.org/10.1016/j.vetimm.2007.05.014

PMID: 17658617

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Abstract

n − 3 fatty acids

CFSE

PGE2

Lipid peroxidation

EPA

DHA

Lymphocyte proliferation and peripheral blood mononuclear cell (PBMC) production of PGE2 were assayed in 15 healthy dogs fed a basal diet supplemented with either sunflower oil (Group Sunflower oil), sunflower oil and menhaden fish oil (Group Fish oil), or sunflower oil and menhaden fish oil plus α-tocopherol acetate for 12 weeks (Group Fish oil + E). Lymphocyte proliferation was determined by a flow cytometric technique utilizing the fluorochrome carboxyfluorescein diacetate succinimidyl ester (CFSE). The PBMC supernatant PGE2 concentration was assayed using a competitive enzyme-linked immunoassay. Group Fish oil had a significant decrease in lymphocyte proliferation at week 12. PBMC production of PGE2 was decreased in all three groups but only significantly reduced in groups receiving fish oil supplementation. Based on these results, this level of fish oil supplementation appears to suppress the lymphoproliferative response in healthy, young dogs but this response can be attenuated by high levels of dietary vitamin E supplementation. Furthermore, fish oil-induced reduction in lymphocyte proliferation appears to manifest through a PGE2-independent mechanism and is not associated with increased lipid peroxidation.

Details

Title: Effects of dietary fish oil and vitamin E supplementation on canine lymphocyte proliferation evaluated using a flow cytometric technique
Authors/Creators: Casey J. LeBlanc - Louisiana State University
Marilyn A. Dietrich - Louisiana State University
David W. Horohov - Louisiana State University
John E. Bauer - Comparative Nutrition Research Laboratory, Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA
Giselle Hosgood - Western Veterinary Specialist Centre 1802, 10th Avenue SW Calgary, Alberta T3C 0J8, Canada
Glenna E. Mauldin - Western Veterinary Specialist Centre 1802, 10th Avenue SW Calgary, Alberta T3C 0J8, Canada
Publication Details: Veterinary immunology and immunopathology, Vol.119(3), pp.180-188
Publisher: Elsevier B.V
Identifiers: 991005592659707891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.68 Lipids; 1.68.256 Fatty Acids
Web Of Science research areas: Immunology; Veterinary Sciences
ESI research areas: Plant & Animal Science