Journal article
Effects of glutathione reductase inhibition on cellular thiol redox state and related systems
Archives of Biochemistry and Biophysics, Vol.485(1), pp.56-62
2009
Abstract
Although inhibition of glutathione reductase (GR) has been demonstrated to cause a decrease in reduced glutathione (GSH) and increase in glutathione disulfide (GSSG), a systematic study of the effects of GR inhibition on thiol redox state and related systems has not been noted. By employing a monkey kidney cell line as the cell model and 2-acetylamino-3-[4-(2-acetylamino-2-carboxy-ethylsulfanylthio carbonylamino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA) as a GR inhibitor, an investigation of the effects of GR inhibition on cellular thiol redox state and related systems was conducted. Our study demonstrated that, in addition to a decrease in GSH and increase in GSSG, 2-AAPA increased the ratios of NADH/NAD+ and NADPH/NADP+. Significant protein glutathionylation was observed. However, the inhibition did not affect the formation of reactive oxygen species or expression of antioxidant defense enzyme systems [GR, glutathione peroxidase, catalase, and superoxide dismutase] and enzymes involved in GSH biosynthesis [γ-glutamylcysteine synthetase and glutathione synthetase].
Details
- Title
- Effects of glutathione reductase inhibition on cellular thiol redox state and related systems
- Authors/Creators
- Y. Zhao (Author/Creator)T. Seefeldt (Author/Creator)W. Chen (Author/Creator)X. Wang (Author/Creator)D. Matthees (Author/Creator)Y. Hu (Author/Creator)X. Guan (Author/Creator)
- Publication Details
- Archives of Biochemistry and Biophysics, Vol.485(1), pp.56-62
- Publisher
- Elsevier
- Identifiers
- 991005542497307891
- Copyright
- © 2009 Elsevier Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.127 Molecular & Cell Biology - Pharmacology
- 1.127.973 Thiol-Disulfide Systems
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- ESI research areas
- Biology & Biochemistry