Journal article
Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro
Reproduction, Fertility and Development, Vol.1(2), pp.89-98
1989
Abstract
Day-4 mouse embyros grew well in culture media supplemented with macromolecular components of uterine fluids recovered on day 3, 4 or 5 of pregnancy and pseudopregnancy. Addition of these components to media during a 2-h pulse culture had no significant effect on the incorporation of glucose carbon by morulae/early blastocysts. However, various fractions of uterine luminal macromolecules significantly increased the turnover of glucose carbon incorporated into acid-soluble and acid-insoluble glycogen, into nucleic acids and into proteins during a 24-h chase culture. These effects were due mainly to components with a molecular weight between 1000 and 10 000 Da and the activity was most marked in fluids collected on day 5 of pregnancy or pseudopregnancy. Oxidation of glucose during a 4-h incubation was inhibited in the presence of certain uterine macromolecules but most consistently by the large molecular weight component (>300 000 Da). Some differences were noted in the inhibitory activity of macromolecules obtained from pregnant and pseudopregnant sources. There was little evidence of an effect of uterine-fluid components on lactate production from glucose.
Details
- Title
- Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro
- Authors/Creators
- N.K. Khurana (Author/Creator) - Murdoch UniversityR.G. Wales (Author/Creator)
- Publication Details
- Reproduction, Fertility and Development, Vol.1(2), pp.89-98
- Publisher
- CSIRO Publishing
- Identifiers
- 991005543723007891
- Copyright
- © CSIRO 1989.
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Developmental Biology
- Reproductive Biology
- Zoology
- ESI research areas
- Plant & Animal Science