Journal article
Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro
Reproduction, Vol.79(1), pp.267-273
1987
Abstract
The addition of progesterone (10−7 to 10−5 M) and/or oestradiol (10−10 M) during 24-h chase culture of pulse-labelled morulae–early blastocysts did not affect the degradation of radiolabelled glycogen or other biochemical fractions. The presence of a high concentration of progesterone (10−5M) during 5-h pulse culture significantly inhibited incorporation of substrate carbon from [U-14C]glucose into both the acid-soluble and acid-insoluble glycogen fractions, but had no effect on non-glycogen fractions. Catabolic utilization of glucose as estimated by the rate of carbon dioxide and lactate production was not affected by the presence of progesterone (10−7 to 10− 5M), oestradiol (10−10 to 10−8M) or a combination of both. The results indicate that ovarian steroids at expected physiological concentrations do not directly influence embryonic energy metabolism.
Details
- Title
- Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro
- Authors/Creators
- N.K. Khurana (Author/Creator)R.G. Wales (Author/Creator)
- Publication Details
- Reproduction, Vol.79(1), pp.267-273
- Publisher
- Society for Reproduction and Fertility
- Identifiers
- 991005543768007891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
Metrics
28 Record Views