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Effects of oral Resveratrol supplementation on development of hemorrhagic shock and acute kidney injury: A randomized double-blinded controlled canine study
Journal article   Peer reviewed

Effects of oral Resveratrol supplementation on development of hemorrhagic shock and acute kidney injury: A randomized double-blinded controlled canine study

K.M. Ho, J. Davis, A.L. Raisis, G. Rossi and R. Cianciolo
Shock, Vol.47(Supp. 1 6S), pp.74-74
40th Annual Conference on Shock (Fort Lauderdale, FL, 03/06/2017–06/06/2017)
06/2017
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Abstract

Objectives: To assess whether oral resveratrol supplementation is useful to reduce risk of developing severe hypotension and acute kidney injury (AKI) from hemorrhage. Methods: Twelve greyhounds were randomized to receive either oral resveratrol (10mg/kg) or placebo for 7 days prior to inducing hemorrhagic shock – aiming at mean arterial pressure (MAP) target<40 mmHg – for 1 hour, followed by resuscitation with intravenous gelatine solution to maintain MAP >60 mmHg for 3hours. Dogs were euthanized and renal tissue was examined for features of AKI at the end of the study. All investigators including the histopathologist were blinded to the treatment allocation. Linear mixed model was used to assess the effect of resveratrol on renal biomarker profiles, after adjusting for the amount of blood loss. Results: A substantial larger amount of blood loss was required to achieve hemorrhagic shock after resveratrol supplementation compared to placebo (67 vs. 51ml/kg, difference 16 ml/kg, 95% confidence difference [CI] 10–21; p<0.001), and to result in similar changes in MAP, cardiac index, and systemic oxygen extraction (Figure 1). Serum cystatin concentrations – a glomerular injury biomarker - were substantially higher in the controls compared to the dogs in the resveratrol group (difference 13.3 ng/ml, 95%CI 12.6–14.0; P<0.001) after adjusting for the amount of blood loss needed to induce hemorrhagic shock. The urine renal tubular biomarkers including Neutrophil gelatinase-associated lipocalin (NGAL), Kidney Injury Molecule-1 (KIM-1), and Clusterin were not significantly different between the two groups. Conclusions: Oral resveratrol treatment increased the amount of blood loss needed to induce hemorrhagic shock, and appeared to attenuate the development glomerular injury from hemorrhagic shock.

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