Journal article
Effects of potential probiotic Enterococcus casseliflavus (EC-001) on growth performance, immunity, and resistance to Aeromonas hydrophila infection in common Carp (Cyprinus carpio)
Probiotics and Antimicrobial Proteins
2021
Abstract
The effects of different levels of dietary Enterococcus casseliflavus (EC-001), as a potential probiotic, were investigated on the growth performance, hemato-biochemical parameters, immune responses, and resistance to Aeromonas hydrophila infection in common carp (Cyprinus carpio) fingerlings. Accordingly, fish (N = 720; 12.0 ± 0.5 g) were distributed into four treatments receiving different dietary levels of E. casseliflavus, EC-001 (0 [control], 1 × 107, 108, and 109 CFU g−1 feed), for 8 weeks. The fish fed with a diet containing 109 CFU g−1 showed the highest weight gain and specific growth rate, along with the lowest feed conversion ratio, compared with the control group (P < 0.05). Red and white blood cells, hemoglobin, hematocrit, neutrophils, and monocytes significantly increased in the fish fed with 1 × 108 and 109 CFU g−1 (P < 0.05). Dietary inclusion of 1 × 108 and 109 CFU g−1 significantly increased serum total protein, albumin, and immunoglobulin content (P < 0.05). Feeding the fish with 1 × 109 CFU g−1 resulted in a significant increase in serum and skin mucus lysozyme activity compared with the other groups (P < 0.05). Complement component 3 and skin mucus protease activity were also significantly higher in all the fish treated with dietary E. casseliflavus (EC-001) compared with the control group (P < 0.05). The cumulative mortality in the treated fish was lower (ranging from 10 to 22%) than the control group (31%) after challenging the fish with A. hydrophila infection, while the fish fed with E. casseliflavus (EC-001) at 1 × 109 CFU g−1 exhibited the lowest mortality rate (P < 0.05). In conclusion, our results revealed the potential probiotic effects of E. casseliflavus (EC-001) for enhancing growth performance, immunity, and disease resistance of common carp.
Details
- Title
- Effects of potential probiotic Enterococcus casseliflavus (EC-001) on growth performance, immunity, and resistance to Aeromonas hydrophila infection in common Carp (Cyprinus carpio)
- Authors/Creators
- H. Akbari (Author/Creator) - Islamic Azad University, Science and Research BranchS.P.H. Shekrabi (Author/Creator)M. Soltani (Author/Creator) - University of TehranM.S. Mehrgan (Author/Creator) - Islamic Azad University, Science and Research Branch
- Publication Details
- Probiotics and Antimicrobial Proteins
- Publisher
- Springer New York LLC
- Identifiers
- 991005540611307891
- Copyright
- © 2021 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Centre for Sustainable Aquatic Ecosystems; Freshwater Fish Group and Fish Health Unit; Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.2 Marine Biology
- 3.2.116 Aquaculture Nutrition
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Microbiology