Journal article
Effects of prostaglandins E-2 and F-2 on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro
Reproduction, Vol.79(1), pp.275-280
1987
Abstract
Summary. During 5-h culture in the presence of radioactive glucose, PGE-2 (10 μg/ml) significantly inhibited incorporation of glucose into the acid-soluble glycogen pool. PGE-2 at 1 and 10 μg/ml and PGF-2α at 1 μg but not 10 μg/ml stimulated incorporation of glucose into non-glycogen macromolecules during culture. However, the utilization of acid-soluble glycogen and other biochemical pools was not affected by the presence of PGs in the medium during 24-h chase culture of pulse-labelled embryos. Carbon dioxide production was significantly suppressed in the presence of PGs but accumulation of lactate was not affected. The results indicate that PGE-2 and PGF-2α, in physiological concentrations, directly influence the metabolism of glucose by preimplantation embryos.
Details
- Title
- Effects of prostaglandins E-2 and F-2 on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro
- Authors/Creators
- N.K. Khurana (Author/Creator)R.G. Wales (Author/Creator)
- Publication Details
- Reproduction, Vol.79(1), pp.275-280
- Publisher
- Society for Reproduction and Fertility
- Identifiers
- 991005543644207891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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