Logo image
Back
Efficacy of D-glucose-13C6 as a label for amino acid identification and quantification in Mediterranean fruit flies (Diptera: Tephritidae)
Journal article   Peer reviewed

Efficacy of D-glucose-13C6 as a label for amino acid identification and quantification in Mediterranean fruit flies (Diptera: Tephritidae)

H. Al-Khshemawee, M. Agarwal, B. Du, J.O. Yang and Y. Ren
Journal of Entomological Science, Vol.55(1), pp.117-125
2020
DOI: https://doi.org/10.18474/0749-8004-55.1.117
url
Link to Published Version *Subscription may be requiredView

Abstract

The efficacy of D-glucose labeled with 13C6 (D-glucose-13C6) in the study of amino acid metabolism in insects was determined by incorporating either D-glucose-13C6 or unlabeled D-glucose into larval and adult diets of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Feeding treatments were: (a) labeled glucose diet for larvae + unlabeled glucose diet for adults; (b) unlabeled glucose diet for larvae + labeled glucose diet for adults; (c) labeled glucose diet for larvae + labeled glucose diet for adults; and (d) unlabeled glucose diet for larvae + unlabeled glucose diet for adults. Amino acid content of extracts from unmated adults and mated adults was determined with gas chromatography-mass spectrometry (GC-MS). L-alanine, L-aspartic acid, L-cysteine, L-glutamic acid, L-glycine, L-isoleucine, L-leucine, L-methionine, L-proline, L-phenylalanine, L-serine, L-threonine, L-tyrosine, and L-valine were identified from the insect extracts. In the treatment that fed unlabeled glucose larval and adult diets (control), amino acid content was lower after mating than before mating except for L-cysteine (e.g., 13-fold increase). Amino acid content in insects fed D-glucose-13C6 also decreased after mating except for L-valine, L-leucine, L-tyrosine, and L-isoleucine, all of which remained comparatively equal before and after mating, and L-threonine which increased almost 12-fold after mating. Only trace amounts of L-tyrosine and L-isoleucine were detected before and after mating in those fed labeled diets. These results suggest that stable isotopes can be used as labels for insect metabolic studies.

Details

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#13 Climate Action

Source: InCites

Metrics

44 Record Views
1 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.32 Entomology
3.32.1485 Insect Cold Adaptation
Web Of Science research areas
Entomology
ESI research areas
Plant & Animal Science
Logo image