Efficacy of dietary live or heat‐killed Bacillus subtilis in goldfish ( Carassius auratus ) infected with Ichthyophthirius multifiliis

Parisa Shahbazi; Najmeh Sheikhzadeh; Mohamad Ali Nazarpour Siahtan; Amin Keshavarz Ghadimi; Mehdi Soltani; Katayoon Nofouzi; Shalaleh Mousavi; Monireh Khordadmehr; Amin Marandi; Masoumeh Firouzamandi

doi:10.1002/vms3.1183

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Efficacy of dietary live or heat‐killed Bacillus subtilis in goldfish ( Carassius auratus ) infected with Ichthyophthirius multifiliis

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Efficacy of dietary live or heat‐killed Bacillus subtilis in goldfish ( Carassius auratus ) infected with Ichthyophthirius multifiliis

Parisa Shahbazi, Najmeh Sheikhzadeh, Mohamad Ali Nazarpour Siahtan, Amin Keshavarz Ghadimi, Mehdi Soltani, Katayoon Nofouzi, Shalaleh Mousavi, Monireh Khordadmehr, Amin Marandi and Masoumeh Firouzamandi

Veterinary medicine and science, Vol.9(4), pp.1636-1645

2023

DOI: https://doi.org/10.1002/vms3.1183

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Abstract

Background The beneficial effects of Bacillus subtilis on growth, immune response, and disease resistance against various diseases in different fish species have been proved. However, there are no data concerning this probiotic effect on skin mucosal immunity in fish infected with Ichthyophthirius multifiliis (Ich). Ich has a high mortality rate in both edible and ornamental fish and consequently is concerned with heavy economic losses. Objectives Thus, we assessed the efficacy of live and heat-killed B. subtilis on skin immunity and histopathology in goldfish (Carassius auratus) infected with Ich. Methods Goldfish (144 fish, 2.38 g average weight) were stocked in nine glass tanks each in three replicates. Fish were fed 109 CFU g−1 live or heat-killed B. subtilis for 80 days. Results Probiotic administration in both viable and non-viable forms could enhance the growth performance in goldfish. Probiotic therapy also reduced the density of the parasite and histopathological level on skin and gill tissues of the treated fish. Real-time polymerase chain reaction analysis showed a higher expression of lysozyme and tumour necrosis factor-α in the treated groups compared to the control group. Conclusions These data demonstrated the beneficial effect of B. subtilis as probiotic and paraprobiotic on growth performance and disease resistance to Ich infestation in goldfish.

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Title: Efficacy of dietary live or heat‐killed Bacillus subtilis in goldfish ( Carassius auratus ) infected with Ichthyophthirius multifiliis
Authors/Creators: Parisa Shahbazi - University of Tabriz
Najmeh Sheikhzadeh - University of Tabriz
Mohamad Ali Nazarpour Siahtan - University of Tabriz
Amin Keshavarz Ghadimi - University of Tabriz
Mehdi Soltani - Department of Aquatic Animal Health Faculty of Veterinary Medicine University of Tehran Tehran Iran, Centre for Sustainable Aquatic Ecosystems Harry Butler Institute Murdoch University Murdoch Australia
Katayoon Nofouzi - University of Tabriz
Shalaleh Mousavi - University of Tabriz
Monireh Khordadmehr - University of Tabriz
Amin Marandi - University of Tehran
Masoumeh Firouzamandi - University of Tabriz
Publication Details: Veterinary medicine and science, Vol.9(4), pp.1636-1645
Publisher: John Wiley & Sons Ltd.
Identifiers: 991005591968007891
Murdoch Affiliation: Centre for Sustainable Aquatic Ecosystems
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.2 Marine Biology; 3.2.116 Aquaculture Nutrition
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science