Journal article
Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
Organic & Biomolecular Chemistry, Vol.7(7), pp.1404-1409
2009
Abstract
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.
Details
- Title
- Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
- Authors/Creators
- R.N. Veedu (Author/Creator) - University of Southern DenmarkB. Vester (Author/Creator) - University of Southern DenmarkJ. Wengel (Author/Creator) - University of Southern Denmark
- Publication Details
- Organic & Biomolecular Chemistry, Vol.7(7), pp.1404-1409
- Publisher
- Royal Society of Chemistry
- Identifiers
- 991005542819107891
- Copyright
- © 2009 Royal Society of Chemistry
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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