Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)

R. Yang; B. Brice; A. Elliot; E. Lee; U. Ryan

doi:10.1016/j.exppara.2015.04.019

Back

Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)

Journal article

Open access

Peer reviewed

Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)

R. Yang, B. Brice, A. Elliot, E. Lee and U. Ryan

Experimental Parasitology, Vol.154, pp.75-81

2015

DOI: https://doi.org/10.1016/j.exppara.2015.04.019

Files and links (2)

pdf

eimeria_collieie_n._sp.pdfDownload View

Author’s VersionCC BY-NC-ND V4.0, Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

A new species, Eimeria collieie n. sp., is described from the western long-necked turtle (Chelodina colliei). Sporulated oocysts (n = 35) are spherical to subspherical, with colourless single layer oocyst wall, 0.6 ± 0.2 (0.4-0.7) μm thick. Oocyst with elongated ellipsoid sporocysts. Oocyst length, 29.8 ± 0.4 (28.2-31.0) μm; oocyst width, 29.4 ± 0.3 (28.0-30.8) μm; oocyst length/width (L/W) ratio, 1.0 ± 0.03 (1.0-1.05). Micropyle, oocyst residuum and polar granule were absent. Sporocysts with sporocyst residuum and 2 sporozoites. Sporocyst length, 21.6 ± 0.4 (21.2-22.0) μm; sporocyst width, 6.0 ± 0.3 (5.7-6.3) μm; sporocyst L/W ratio, 3.6 ± 0.2 (3.4-3.8). Stieda, parastieda and substieda bodies were absent. Sporozoite length, 14.0 ± 0.2 (13.8-14.2) μm; sporozoite width, 2.6 ± 0.2 (2.4-2.8) μm; sporozoite L/W ratio, 5.46 ± 0.10 (5.4-5.6). Molecular analysis was conducted at three loci: the 18S and 28S ribosomal RNA (rRNA), and the mitochondrial cytochrome oxidase gene (COI). At the 18S rRNA locus, E. collieie n. sp. shared 96.4% and 98.3% genetic similarity to E. ranae (GenBank accession number: EU717219) and E. arnyi (AY613853) respectively. At the 28S rRNA locus, E. collieie n. sp. shared 91.6% genetic similarity to E. papillata (GenBank accession number: GU593706) and phylogenetic analysis at this locus placed E. collieie n. sp. in aseparateclade. At the COI locus, E. collieie n. sp. shared 92.7% genetic similarity to Eimeria setonicis (GenBankaccession number: KF225638) from a quokka (Setonix brachyurus) in Western Australia. Reptile-derived sequences were not available for the 28S rRNA and the COI loci. Based on morphological and molecular data, this isolate is a new species of coccidian parasite that, to date, has only been found in western long-necked turtles.

Details

Title: Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)
Authors/Creators: R. Yang (Author/Creator) - Murdoch University
B. Brice (Author/Creator) - Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia
A. Elliot (Author/Creator)
E. Lee (Author/Creator) - Murdoch University
U. Ryan (Author/Creator) - Murdoch University
Publication Details: Experimental Parasitology, Vol.154, pp.75-81
Publisher: Academic Press
Identifiers: 991005542463707891
Copyright: © 2015 Published by Elsevier Inc.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

187 File views/ downloads

42 Record Views

7 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 1 Clinical & Life Sciences; 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis; 1.217.1890 Coccidiosis
Web Of Science research areas: Parasitology
ESI research areas: Microbiology