Journal article
Electroporation of cells
Physiologia Plantarum, Vol.79(1), pp.168-172
1990
Abstract
By measuring uptake of the membrane impermeable dye. phenosafranine, it can be shown that the plasma membrane of intact cells within cell aggregates can be reversibly permeabilized by electroporation. However, the plant cell wall is a barrier to DNA uptake by intact cells, although under certain circumstances expression of DNA, electroporated into intact cells, can be demonstrated. The level of expression is about 20–50 times lower than that obtained by electroporation of protoplasts, and depends on cell wall properties and pretreatments of cell aggregates. In contrast, efficient transformation of whole cells of bacteria and yeasts can be achieved by electroporation. Factors which influence DNA transfer into whole plant cells and the possibility of stable transformation are discussed.
Details
- Title
- Electroporation of cells
- Authors/Creators
- K. Lindsey (Author/Creator) - The Hertz CorporationM.G.K. Jones (Author/Creator) - The Hertz Corporation
- Publication Details
- Physiologia Plantarum, Vol.79(1), pp.168-172
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005541293707891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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