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Elevated serum thymidine kinase activity in canine splenic hemangiosarcoma
Journal article   Peer reviewed

Elevated serum thymidine kinase activity in canine splenic hemangiosarcoma

D.H. Thamm, D.A. Kamstock, C.R. Sharp, S.I. Johnson, E. Mazzaferro, L.V. Herold, S.M. Barnes, K. Winkler and K.A. Selting
Veterinary and Comparative Oncology, Vol.10(4), pp.292-302
2011
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Abstract

Thymidine kinase 1 (TK1) is a soluble biomarker associated with DNA synthesis. This prospective study evaluated serum TK1 activity in dogs presenting with hemoabdomen and a splenic mass. An ELISA using azidothymidine as a substrate was used to evaluate TK1 activity. Sixty‐two dogs with hemoabdomen and 15 normal controls were studied. Serum TK1 activity was significantly higher in dogs with hemangiosarcoma (HSA) than in normal dogs (mean ± SEM = 17.0 ± 5.0 and 2.01 ± 0.6, respectively), but not dogs with benign disease (mean ± SEM = 10.0 ± 3.3). Using a cut‐off of 6.55 U/L, TK activity demonstrated a sensitivity of 0.52, specificity of 0.93, positive predictive value of 0.94 and negative predictive value of 0.48 for distinguishing HSA versus normal. When interval thresholds of <1.55 and >7.95 U/L were used together, diagnostic utility was increased. Serum TK1 evaluation may help to discriminate between benign disease and HSA in dogs with hemoabdomen and a splenic mass.

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Collaboration types
Domestic collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.232 Veterinary Sciences
3.232.1120 Veterinary Oncology
Web Of Science research areas
Veterinary Sciences
ESI research areas
Plant & Animal Science
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