Abstract
The CRISPR/Cas system consists of RNA-guided nucleases whose target specificity is directed by Watson-Crick base pairing of target loci with single guide-RNA to induce desired edits. CRISPR-associated proteins and other engineered nucleases are opening new avenues of research in crops to induce heritable mutations. Here, we review the diversity of CRISPR-associated proteins and strategies to deregulate genome-edited (GE) crops by considering them to be close to natural processes. This technology ensures yield without penalties, advances plant breeding, and guarantees manipulation of the genome for desirable traits. DNA-free and off-target free GE crops with defined characteristics can help to achieve sustainable global food security under a changing climate but need alignment of international regulations to operate in the existing supply chains.