Journal article
Enhanced nodulation and symbiotic effectiveness of Medicago truncatula when co-inoculated with Pseudomonas fluorescens WSM3457 and Ensifer (Sinorhizobium) medicae WSM419
Plant and Soil, Vol.348(1-2), pp.245-254
2011
Abstract
Aims: Low numbers of rhizobia in soil or inoculants delay nodulation and decrease symbiotic legume productivity. This study investigated the effect of co-inoculation with a helper bacterium, Pseudomonas fluorescens WSM3457 on the Medicago truncatula - Ensifer (Sinorhizobium) medicae WSM419 symbiosis challenged by a low inoculum dose. Methods: In a glasshouse experiment the effect of co-inoculation with WSM3457 on the kinetics of nodule initiation and development was assessed 5,7,10,14,1721, and 42 days after inoculation of M. truncatula cv. Caliph with 103 cells/plant of E. medicae WSM419. Results: Co-inoculated plants had enhanced rate of nodule initiation and development, greater numbers of larger crown nodules, and by day 42 accumulated more N than plants inoculated with E. medicae WSM419 alone. Nodule development was altered by co-inoculation. Approximately 25% of nodule initials on co-inoculated plants formed in closely associated pairs, young nodules were larger with multiple meristems and developed into cluster-like multi-lobed nodules compared to those on WSM419 inoculated plants. Molecular typing showed WSM3457 occupied a significant proportion of root nodules on co-inoculated plants. Conclusion: Co-inoculation with P. fluorescens WSM3457 enhanced symbiotic effectiveness of M. truncatula when inoculated with a low inoculum dose of E. medicae WSM419.
Details
- Title
- Enhanced nodulation and symbiotic effectiveness of Medicago truncatula when co-inoculated with Pseudomonas fluorescens WSM3457 and Ensifer (Sinorhizobium) medicae WSM419
- Authors/Creators
- S.L. Fox (Author/Creator)G.W. O'Hara (Author/Creator)L. Bräu (Author/Creator)
- Publication Details
- Plant and Soil, Vol.348(1-2), pp.245-254
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005542636407891
- Copyright
- © 2011 The Author(s).
- Murdoch Affiliation
- Centre for Rhizobium Studies; School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Agronomy
- Plant Sciences
- Soil Science
- ESI research areas
- Agricultural Sciences