Environmental contamination with Clostridioides (Clostridium) difficile in Vietnam

Peng An Khun; Long Duc Phi; Huong Thi Thu Bui; Nguyen Thi Bui; Quyen Thi Huyen Vu; Luong Duy Trinh; Deirdre A. Collins; Thomas Riley

doi:10.1093/jambio/lxad118

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Environmental contamination with Clostridioides (Clostridium) difficile in Vietnam

Journal article

Open access

Peer reviewed

Environmental contamination with Clostridioides (Clostridium) difficile in Vietnam

Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Nguyen Thi Bui, Quyen Thi Huyen Vu, Luong Duy Trinh, Deirdre A. Collins and Thomas Riley

Journal of applied microbiology, Vol.134(6)

2023

DOI: https://doi.org/10.1093/jambio/lxad118

PMID: 37296244

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Abstract

Biotechnology & Applied Microbiology

Life Sciences & Biomedicine

Microbiology

Science & Technology

Aims To investigate the prevalence, molecular type, and antimicrobial susceptibility of Clostridioides difficile in the environment in Vietnam, where little is known about C. difficile. Methods and results Samples of pig faeces, soils from pig farms, potatoes, and the hospital environment were cultured for C. difficile. Isolates were identified and typed by polymerase chain reaction (PCR) ribotyping. The overall prevalence of C. difficile contamination was 24.5% (68/278). Clostridioides difficile was detected mainly in soils from pig farms and hospital soils, with 70%-100% prevalence. Clostridioides difficile was isolated from 3.4% of pig faecal samples and 5% of potato surfaces. The four most prevalent ribotypes (RTs) were RTs 001, 009, 038, and QX574. All isolates were susceptible to metronidazole, fidaxomicin, vancomycin, and amoxicillin/clavulanate, while resistance to erythromycin, tetracycline, and moxifloxacin was common in toxigenic strains. Clostridioides difficile RTs 001A(+)B(+)CDT(-) and 038A(-)B(-)CDT(-) were predominantly multidrug resistant. Conclusions Environmental sources of C. difficile are important to consider in the epidemiology of C. difficile infection in Vietnam, however, contaminated soils are likely to be the most important source of C. difficile. This poses additional challenges to controlling infections in healthcare settings.

Details

Title: Environmental contamination with Clostridioides (Clostridium) difficile in Vietnam
Authors/Creators: Peng An Khun - The University of Western Australia
Long Duc Phi - Thai Binh University of Medicine and Pharmacy
Huong Thi Thu Bui - Thai Binh University of Medicine and Pharmacy
Nguyen Thi Bui - Children's Hospital 1
Quyen Thi Huyen Vu - Children's Hospital 1
Luong Duy Trinh - Children's Hospital 1
Deirdre A. Collins - The University of Western Australia
Thomas Riley - Murdoch University, Centre for Biosecurity and One Health
Publication Details: Journal of applied microbiology, Vol.134(6)
Publisher: Oxford University Press
Number of pages: 10
Grant note: National Health and Medical Research Council; National Health and Medical Research Council (NHMRC) of Australia University of Western Australia
Identifiers: 991005593761907891
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.120 Inflammatory Bowel Diseases & Infections; 1.120.1133 Clostridium Infections
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Biology & Biochemistry