Journal article
Enzymatic Recognition of 2′-Modified Ribonucleoside 5′-Triphosphates: Towards the Evolution of Versatile Aptamers
ChemBioChem, Vol.13(1), pp.19-25
2011
Abstract
The quest for effective, selective and nontoxic nucleic-acid-based drugs has led to designing modifications of naturally occurring nucleosides. A number of modified nucleic acids have been made in the past decades in the hope that they would prove useful in target-validation studies and therapeutic applications involving antisense, RNAi, aptamer, and ribozyme-based technologies. Since their invention in the early 1990s, aptamers have emerged as a very promising class of therapeutics, with one drug entering the market for the treatment of age-related macular degeneration. To combat the limitations of aptamers containing naturally occurring nucleotides, chemically modified nucleotides have to be used. In order to apply modified nucleotides in aptamer drug development, their enzyme-recognition capabilities must be understood. For this purpose, several modified nucleoside 5′-triphosphates were synthesized and investigated as substrates for various enzymes. Herein, we review studies on the enzyme-recognition of various 2′-sugar-modified NTPs that were carried out with a view to their effective utilization in SELEX processes to generate versatile aptamers.
Details
- Title
- Enzymatic Recognition of 2′-Modified Ribonucleoside 5′-Triphosphates: Towards the Evolution of Versatile Aptamers
- Authors/Creators
- L.H. Lauridsen (Author/Creator)J.A. Rothnagel (Author/Creator)R.N. Veedu (Author/Creator)
- Publication Details
- ChemBioChem, Vol.13(1), pp.19-25
- Publisher
- Wiley
- Identifiers
- 991005543211307891
- Copyright
- © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.988 Oligonucleotide Modifications
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Medicinal
- ESI research areas
- Biology & Biochemistry