Journal article
Enzymatic polymerisation involving 2′-amino-LNA nucleotides
Bioorganic & Medicinal Chemistry Letters, Vol.22(10), pp.3522-3526
2012
Abstract
The triphosphate of the thymine derivative of 2′-amino-LNA (2′-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion® HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2′-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2′-Amino-LNA-T in the template and 2′-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2′-amino-LNA-T nucleotides.
Details
- Title
- Enzymatic polymerisation involving 2′-amino-LNA nucleotides
- Authors/Creators
- M.W. Johannsen (Author/Creator)R.N. Veedu (Author/Creator)A.S. Madsen (Author/Creator)J. Wengel (Author/Creator)
- Publication Details
- Bioorganic & Medicinal Chemistry Letters, Vol.22(10), pp.3522-3526
- Publisher
- Elsevier BV
- Identifiers
- 991005543337307891
- Copyright
- © 2012 Published by Elsevier Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.988 Oligonucleotide Modifications
- Web Of Science research areas
- Chemistry, Medicinal
- Chemistry, Organic
- ESI research areas
- Chemistry