Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

T. Højland; R.N. Veedu; B. Vester; J. Wengel

doi:10.4161/adna.19272

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Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

Journal article

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Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

T. Højland, R.N. Veedu, B. Vester and J. Wengel

Artificial DNA: PNA & XNA, Vol.3(1), pp.14-21

2012

DOI: https://doi.org/10.4161/adna.19272

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Abstract

We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5′-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°Nm, Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucleotides. In addition it was found that primer extension can be achieved using templates containing one α-L-LNA nucleotide.

Details

Title: Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides
Authors/Creators: T. Højland (Author/Creator) - University of Southern Denmark
R.N. Veedu (Author/Creator) - The University of Queensland
B. Vester (Author/Creator) - University of Southern Denmark
J. Wengel (Author/Creator) - University of Southern Denmark
Publication Details: Artificial DNA: PNA & XNA, Vol.3(1), pp.14-21
Publisher: Taylor & Francis
Identifiers: 991005543041607891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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