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Enzymatically active colloidal crystal arrays
Journal article   Peer reviewed

Enzymatically active colloidal crystal arrays

A. Yu and Z. Liang
Journal of Colloid and Interface Science, Vol.330(1), pp.144-148
2009
DOI: https://doi.org/10.1016/j.jcis.2008.10.030
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Abstract

We report the construction of three-dimensional (3D) colloidal crystal arrays (CCA) and hollow colloidal crystal arrays (HCCA) derived from the self-assembly of polyelectrolytes (PE)-coated polystyrene (PS) particles and their use as models of high surface area systems to immobilize peroxidase (POD). POD molecules could infiltrate into the deep layers of CCA and HCCA through their interconnected pores and strongly adsorbed at the PE shell of the colloidal particles. And the total enzyme loading amount and bioactivities increased linearly with the thickness of the CCA till ca. 10 μm. Compared with flat substrates with the same geometrical area, CCA and HCCA exhibit much higher enzyme loading abilities (~43 and 53 times respectively) and the resulting bioactivities (~35 and 41 times respectively) due to their inherently higher surface area and 3D interconnected porous structures. In addition, HCCA could load approximately 30% more POD than CCA because some POD molecules could infiltrate into the interior of the hollow capsule under salt condition.

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Source: InCites

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15 Record Views
4 Times Cited - Web of Science

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Collaboration types
Domestic collaboration
Citation topics
2 Chemistry
2.53 Polymers & Macromolecules
2.53.1624 Polyelectrolyte Multilayers
Web Of Science research areas
Chemistry, Physical
ESI research areas
Chemistry
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