Journal article
Enzymic assay of 10−7 to 10−14 moles of sucrose in plant tissues
Plant Physiology, Vol.60(3), pp.379-383
1977
Abstract
Procedures are described for measuring sucrose in plant extracts or freeze-dried tissue in the range between 10−7 and 10−14 moles. The method is based on the destruction of pre-existing glucose and fructose, followed by the hydrolysis of sucrose and reduction of NADP+ by a series of coupled enzymic reactions. Depending on the sensitivity required, the NADPH is determined directly with a spectrophotometer or a fluorometer, or is amplified as much as 30,000 times before fluorometric assay. The procedures suggested for the macro level are simpler than current methods, and those suggested for microanalysis are several orders of magnitude more sensitive.
With this technique, single palisade parenchyma cells and single spongy parenchyma cells of Vicia faba leaflets were each found to contain about 2.2 pmoles of sucrose.
Details
- Title
- Enzymic assay of 10−7 to 10−14 moles of sucrose in plant tissues
- Authors/Creators
- M.G.K. Jones (Author/Creator)W.H. Outlaw (Author/Creator)O.H. Lowry (Author/Creator)
- Publication Details
- Plant Physiology, Vol.60(3), pp.379-383
- Publisher
- American Society of Plant Biologists
- Identifiers
- 991005542019507891
- Copyright
- © 1977 American Society of Plant Biologists
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
Metrics
45 Record Views