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Epidemiological survey and risk factor analysis of 14 potential pathogens in Golden Snub-Nosed Monkeys at Shennongjia National Nature Reserve, China
Journal article   Open access   Peer reviewed

Epidemiological survey and risk factor analysis of 14 potential pathogens in Golden Snub-Nosed Monkeys at Shennongjia National Nature Reserve, China

Mingpu Qi, Qiankun Wang, Yu Wang, Yingyu Chen, Changmin Hu, Wanji Yang, Feng Wu, Tianpeng Huang, Ali Sobhy Dawood, Muhammad Zubair, …
Pathogens (Basel), Vol.12(3), Art. 483
2023
DOI: https://doi.org/10.3390/pathogens12030483
PMID: 36986405
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Published1.96 MBDownloadView
CC BY V4.0,  Open Access

Abstract

adenovirus cytomegalovirus epidemiology golden snub-nosed monkey hepatitis A virus macacine herpesvirus 1 simian foamy virus tuberculosis
Golden snub-nosed monkeys (Rhinopithecus roxellanae) belong to Class A, the highest level of endangered primate species. Exploring the infection status of potential pathogens in golden snub-nosed monkeys is important for controlling associated diseases and protecting this species. The objective of this study was to investigate the seroprevalence for a number of potential pathogens and the prevalence of fecal adenovirus and rotavirus. A total of 283 fecal samples were collected from 100 golden snub-nosed monkeys in December 2014, June 2015, and January 2016; 26 blood samples were collected from 26 monkeys in June 2014, June 2015, January 2016 and November 2016 at Shennongjia National Reserve in Hubei, China. The infection of 11 potential viral diseases was examined serologically using an Indirect Enzyme-linked Immunosorbent Assay (iELISA) and Dot Immunobinding Assays (DIA), while the whole blood IFN-γ in vitro release assay was used to test tuberculosis (TB). In addition, fecal Adenovirus and Rotavirus were detected using Polymerase Chain Reaction (PCR). As a result, the Macacine herpesvirus-1 (MaHV-1), Golden snub-nosed monkey cytomegalovirus (GsmCMV), Simian foamy virus (SFV) and Hepatitis A virus (HAV) were detected with the seroprevalence of 57.7% (95% CI: 36.9, 76.6), 38.5% (95% CI: 20.2, 59.4), 26.9% (95% CI: 11.6, 47.8), and 7.7% (95% CI: 0.0, 84.2), respectively. Two fecal samples tested positive for Adenovirus (ADV) by PCR, with a prevalence of 0.7% (95% CI: 0.2, 2.5), and further, the amplification products were sequenced. Phylogenetic analysis revealed that they belonged to the HADV-G group. However, other pathogens, such as Coxsackievirus (CV), Measles virus (MeV), Rotavirus (RV), Simian immunodeficiency virus (SIV), Simian type D retroviruses (SRV), Simian-T-cell lymphotropic virus type 1 (STLV-1), Simian varicella virus (SVV), Simian virus 40 (SV40) and Mycobacterium tuberculosis complex (TB) were negative in all samples. In addition, a risk factor analysis indicated that the seroprevalence of MaHV-1 infection was significantly associated with old age (≥4 years). These results have important implications for understanding the health status and conservation of the endangered golden snub-nosed monkey population at Shennongjia Nature Reserve.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
1 Clinical & Life Sciences
1.161 Virology - Identification & Sequencing
1.161.711 Cytomegalovirus Infections
Web Of Science research areas
Microbiology
ESI research areas
Microbiology
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