Journal article
Evaluation and improvement of parasitological tests for Trypanosoma evansi infection
Veterinary Parasitology, Vol.102(4), pp.291-297
2001
Abstract
Research was undertaken to critically evaluate parasitological tests for the detection of Trypanosoma evansi in blood. The relative sensitivity of mouse inoculation (MI), the haematocrit centrifugation technique (HCT) and a modified miniature anion-exchange centrifugation technique (MAECT) were compared using blood and buffy coat. The effect that storage of blood prior to inoculation into mice has on the reliability of the MI test was also evaluated. The tests may be ranked in increasing order of sensitivity: HCT, MAECT with whole blood, MI with whole blood, MAECT with buffy coat and MI with buffy coat. The latter was able to detect 1.25 T. evansi per 4 ml of blood. The reliability of the MI test was not reduced with storage of blood containing at least 25 T. evansi per ml for up to 21 h prior to inoculation into mice. These results demonstrate that sensitivity of the MI and MAECT are increased approximately 10-fold through the use of buffy coat in place of whole blood. Although, the MI is marginally more sensitive MAECT is better suited to field use.
Details
- Title
- Evaluation and improvement of parasitological tests for Trypanosoma evansi infection
- Authors/Creators
- S.A. Reid (Author/Creator) - Australian Institute of Tropical Health and MedicineA. Husein (Author/Creator) - Research Institute for Veterinary Science, Jl Re Martadinata 30, Bogor 16114, IndonesiaD.B. Copeman (Author/Creator) - Australian Institute of Tropical Health and Medicine
- Publication Details
- Veterinary Parasitology, Vol.102(4), pp.291-297
- Publisher
- Elsevier BV
- Identifiers
- 991005544991407891
- Copyright
- © 2001 Elsevier Science B.V.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.261 Parasitology - Trypanosoma & Leishmania
- 1.261.596 Trypanosoma Biology
- Web Of Science research areas
- Parasitology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science