Evaluation of (-)-epicatechin metabolites as recovery biomarker of dietary flavan-3-ol intake

Javier I Ottaviani; Reedmond Fong; Jennifer Kimball; Jodi L Ensunsa; Nicola Gray; Anna Vogiatzoglou; Abigail Britten; Debora Lucarelli; Robert Luben; Philip B Grace; Deborah H Mawson; Amy Tym; Antonia Wierzbicki; A David Smith; Nicholas J Wareham; Nita G Forouhi; Kay-Tee Khaw; Hagen Schroeter; Gunter G C Kuhnle

doi:10.1038/s41598-019-49702-z

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Evaluation of (-)-epicatechin metabolites as recovery biomarker of dietary flavan-3-ol intake

Journal article

Open access

Peer reviewed

Evaluation of (-)-epicatechin metabolites as recovery biomarker of dietary flavan-3-ol intake

Javier I Ottaviani, Reedmond Fong, Jennifer Kimball, Jodi L Ensunsa, Nicola Gray, Anna Vogiatzoglou, Abigail Britten, Debora Lucarelli, Robert Luben, Philip B Grace, …

Scientific reports, Vol.9(1), 13108

2019

DOI: https://doi.org/10.1038/s41598-019-49702-z

PMCID: 6739331

PMID: 31511603

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Abstract

Adult

Biomarkers - metabolism

Catechin - metabolism

Cohort Studies

Diet

Female

Flavonoids - pharmacology

Healthy Volunteers

Humans

Male

Middle Aged

Data from dietary intervention studies suggest that intake of (-)-epicatechin mediates beneficial vascular effects in humans. However, population-based investigations are required to evaluate associations between habitual intake and health and these studies rely on accurate estimates of intake, which nutritional biomarkers can provide. Here, we evaluate a series of structurally related (-)-epicatechin metabolites (SREM), particularly (-)-epicatechin-3'-glucuronide, (-)-epicatechin-3'-sulfate and 3'-O-methyl-(-)-epicatechin-5-sulfate (SREM ), as flavan-3-ol and (-)-epicatechin intake. SREM in urine proved to be a specific indicator of (-)-epicatechin intake, showing also a strong correlation with the amount of (-)-epicatechin ingested (R : 0.86 (95% CI 0.8l; 0.92). The median recovery of (-)-epicatechin as SREM in 24 h urine was 10% (IQR 7-13%) and we found SREM in the majority of participants of EPIC Norfolk (83% of 24,341) with a mean concentration of 2.4 ± 3.2 µmol/L. Our results show that SREM are suitable as biomarker of (-)-epicatechin intake. According to evaluation criteria from IARC and the Institute of Medicine, the results obtained support use of SREM as a recovery biomarker to estimate actual intake of (-)-epicatechin.

Details

Title: Evaluation of (-)-epicatechin metabolites as recovery biomarker of dietary flavan-3-ol intake
Authors/Creators: Javier I Ottaviani - Mars (United States)
Reedmond Fong - University of California, Davis
Jennifer Kimball - University of California, Davis
Jodi L Ensunsa - University of California, Davis
Nicola Gray - University of Reading
Anna Vogiatzoglou - Coventry University
Abigail Britten - MRC Epidemiology Unit
Debora Lucarelli - MRC Epidemiology Unit
Robert Luben - University of Cambridge
Philip B Grace - LGC, Newmarket Road, Fordham, UK
Deborah H Mawson - LGC, Newmarket Road, Fordham, UK
Amy Tym - LGC, Newmarket Road, Fordham, UK
Antonia Wierzbicki - LGC, Newmarket Road, Fordham, UK
A David Smith - University of Oxford
Nicholas J Wareham - MRC Epidemiology Unit
Nita G Forouhi - MRC Epidemiology Unit
Kay-Tee Khaw - University of Cambridge
Hagen Schroeter - Mars (United States)
Gunter G C Kuhnle - University of Reading
Publication Details: Scientific reports, Vol.9(1), 13108
Publisher: Springer Nature
Grant note: G0401527 / Medical Research Council MR/N003284/1 / Medical Research Council IS-BRC-1215-20014 / Department of Health MC_UU_12015/1 / Medical Research Council 14136 / Cancer Research UK MC_UU_12015/5 / Medical Research Council G1000143 / Medical Research Council
Identifiers: 991005598620007891
Murdoch Affiliation: Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.16 Phytochemicals; 3.16.28 Antioxidant Activity
Web Of Science research areas: Nutrition & Dietetics
ESI research areas: Multidisciplinary