Journal article
Evaluation of a Commercial Sandwich Enzyme-Linked Immunosorbent Assay for the Quantification of Beta-Casomorphin 7 in Yogurt Using Solid-Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry as the “Gold Standard” Method
Journal of AOAC International, Vol.101(2), pp.515-519
2019
Abstract
This study investigated beta-casomorphin 7 (BCM7) in yogurt by means of LC-tandem MS (MS/MS) and enzyme-linked immunosorbent assay (ELISA) and use LC-MS/MS as the "gold standard" method to evaluate the applicability of a commercial ELISA. The level of BCM7 in milk obtained from ELISA analysis was much lower than that obtained by LC-MS/MS analysis and trended to increase during fermentation and storage of yogurt. Meanwhile, the results obtained from LC-MS/MS showed that BCM7 degraded during stages of yogurt processing, and its degradation may have been caused by X-prolyl dipeptidyl aminopeptidase activity. As a result, the commercial sandwich ELISA kit was not suitable for the quantification of BCM7 in fermented dairy milk.
Details
- Title
- Evaluation of a Commercial Sandwich Enzyme-Linked Immunosorbent Assay for the Quantification of Beta-Casomorphin 7 in Yogurt Using Solid-Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry as the “Gold Standard” Method
- Authors/Creators
- D.D. Nguyen (Author/Creator)F. Busetti (Author/Creator) - Edith Cowan UniversityS.K. Johnson (Author/Creator) - Curtin UniversityV.A. Solah (Author/Creator) - Vietnam National University Ho Chi Minh City
- Publication Details
- Journal of AOAC International, Vol.101(2), pp.515-519
- Publisher
- Oxford University Press
- Identifiers
- 991005540123107891
- Copyright
- © 2019 Journal of AOAC International
- Murdoch Affiliation
- Murdoch University; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.1687 Functional Bioactive Peptides
- Web Of Science research areas
- Chemistry, Analytical
- Food Science & Technology
- ESI research areas
- Agricultural Sciences