Journal article
Evaluation of an antibody-ELISA using five crude antigen preparations for the diagnosis of Trypanosoma evansi infection in cattle
Veterinary Parasitology, Vol.104(1), pp.79-84
2002
Abstract
Attempts were made to improve the accuracy of an antibody-detection ELISA for the detection of Trypanosoma evansi infection in cattle by improving the method of preparation of the crude antigen used. An IgG-ELISA was performed with five different antigen preparations: crude soluble antigen, soluble and insoluble fractions of crude antigen treated with 0.1% formalin and whole formalin-fixed trypanosomes treated with either trypsin or 2-mercaptoethanol. An IgM-ELISA using crude soluble antigen was also performed. Each ELISA was evaluated using serum from 44 Indonesian cattle infected with T. evansi and 262 uninfected cattle from Australia. There was no significant difference between the sensitivity or specificity of the IgG-ELISA using each of the five antigens. The IgM-ELISA using a crude untreated lysate was significantly less sensitive (p<0.05) than the IgG-ELISA using the same antigen, trypsin-treated antigen or the 0.1% formalin-treated soluble antigen (68, 64 and 64%, respectively). These results show that these modifications to the method of producing crude antigens for the Ab-ELISA does not improve the accuracy of diagnosis of T. evansi infection in cattle.
Details
- Title
- Evaluation of an antibody-ELISA using five crude antigen preparations for the diagnosis of Trypanosoma evansi infection in cattle
- Authors/Creators
- S.A. Reid (Author/Creator) - Australian Institute of Tropical Health and MedicineD.B. Copeman (Author/Creator) - Australian Institute of Tropical Health and Medicine
- Publication Details
- Veterinary Parasitology, Vol.104(1), pp.79-84
- Publisher
- Elsevier BV
- Identifiers
- 991005541450107891
- Copyright
- (c) 2002 Elsevier BV
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.261 Parasitology - Trypanosoma & Leishmania
- 1.261.596 Trypanosoma Biology
- Web Of Science research areas
- Parasitology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science