Journal article
Evaluation of four serological techniques to determine the seroprevalence of Neospora caninum in foxes (Vulpes vulpes) and coyotes (Canis latrans) on Prince Edward Island, Canada
Veterinary Parasitology, Vol.145(1-2), pp.51-58
10/04/2007
Abstract
The objectives of this study were (1) to evaluate the performance and agreement of serological assays (ELISA, IFAT, Neospora caninum agglutination test and immunoblot) using reference sera and field sera from foxes and coyotes and (2) to estimate the N. caninum seroprevalence in foxes and coyotes on Prince Edward Island, Canada. With fox and coyote reference sera the test performance of the ELISA, IFAT and IB was excellent (100% sensitivity and specificity). NAT showed a low sensitivity (50%). Serum was collected from 201 coyotes and 271 foxes. The seroprevalence observed in the different assays ranged from 0.5 to 14.0% in coyotes and 1.1 to 34.8% in foxes. The seroprevalence, when taking more than one test positive as cut-off value was 3.3 and 1.1% for coyotes and foxes, respectively. From the N. caninum-positive group, all coyotes were older than 3 years. Agreement among assays (measured as prevalence-adjusted bias-adjusted kappa) using the field sera ranged from 0.17 to 0.97. Best agreement was observed between ELISA and IFAT, poor agreement was observed between NAT and the other assays. Positive agreement was moderate to poor among all assays utilized in this study. Although the seroprevalence observed was low, N. caninum antibodies are present in foxes and coyotes on Prince Edward Island (PEI) and their role in the N. caninum epidemiology needs further study.
Details
- Title
- Evaluation of four serological techniques to determine the seroprevalence of Neospora caninum in foxes (Vulpes vulpes) and coyotes (Canis latrans) on Prince Edward Island, Canada
- Authors/Creators
- W. Wapenaar (Author/Creator) - University of Prince Edward IslandH.W. Barkema (Author/Creator) - University of CalgaryG. Schares (Author/Creator) - Friedrich-Loeffler-InstitutK. Rouvinen-Watt (Author/Creator) - Nova Southeastern UniversityL. Zeijlemaker (Author/Creator) - University of Prince Edward IslandB. Poorter (Author/Creator) - University of Prince Edward IslandR. O’Handley (Author/Creator) - Murdoch UniversityO.C.H. Kwok (Author/Creator) - United States Department of AgricultureJ.P. Dubey (Author/Creator) - United States Department of Agriculture
- Publication Details
- Veterinary Parasitology, Vol.145(1-2), pp.51-58
- Publisher
- Elsevier BV
- Identifiers
- 991005542561107891
- Copyright
- © 2007 Elsevier B.V.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- 1 Clinical & Life Sciences
- 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis
- 1.217.1038 Toxoplasma Gondii
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