Journal article
Evaluation of six presumptive tests for blood, their specificity, sensitivity, and effect on high molecular-weight DNA
Journal of Forensic Sciences, Vol.52(1), pp.102-109
2007
Abstract
Luminol, leuchomalachite green, phenolphthalein, Hemastix®, Hemident™, and Bluestar© are all used as presumptive tests for blood. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. The DNA loci tested were D2S1338 and D19S433. Leuchomalachite green had a sensitivity of 1:10,000, while the remaining tests were able to detect blood to a dilution of 1:100,000. Substances tested include saliva, semen, potato, tomato, tomato sauce, tomato sauce with meat, red onion, red kidney bean, horseradish, 0.1 M ascorbic acid, 5% bleach, 10% cupric sulfate, 10% ferric sulfate, and 10% nickel chloride. Of all the substances tested, not one of the household items reacted with every test; however, the chemicals did. DNA was recovered and amplified from luminol, phenolphthalein, Hemastix®, and Bluestar©, but not from leuchomalachite green or Hemident™.
Details
- Title
- Evaluation of six presumptive tests for blood, their specificity, sensitivity, and effect on high molecular-weight DNA
- Authors/Creators
- S.S. Tobe (Author/Creator) - University of StrathclydeN. Watson (Author/Creator) - International Union of Pure and Applied ChemistryN.N. Daéid (Author/Creator)
- Publication Details
- Journal of Forensic Sciences, Vol.52(1), pp.102-109
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005541084007891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.244 Chemometrics
- 2.244.1784 Forensic Spectroscopy
- Web Of Science research areas
- Medicine, Legal
- ESI research areas
- Clinical Medicine