Journal article
Evaluation of the BD Max Cdiff assay for the detection of toxigenic Clostridium difficile in human stool specimens
Pathology, Vol.47(2), pp.165-168
2015
Abstract
The Becton Dickinson (BD) PCR-based GeneOhm Cdiff assay has demonstrated a high sensitivity and specificity for detecting Clostridium difficile. Recently, the BD Max platform, using the same principles as BD GeneOhm, has become available in Australia. This study aimed to investigate the sensitivity and specificity of BD Max Cdiff assay for the detection of toxigenic C. difficile in an Australian setting. Between December 2013 and January 2014, 406 stool specimens from 349 patients were analysed with the BD Max Cdiff assay. Direct and enrichment toxigenic culture were performed on bioMérieux ChromID C. difficile agar as a reference method. isolates from specimens with discrepant results were further analysed with an in-house PCR to detect the presence of toxin genes. The overall prevalence of toxigenic C. difficile was 7.2%. Concordance between the BD Max assay and enrichment culture was 98.5%. The sensitivity, specificity, positive predictive value and negative predictive value for the BD Max Cdiff assay were 95.5%, 99.0%, 87.5% and 99.7%, respectively, when compared to direct culture, and 91.7%, 99.0%, 88.0% and 99.4%, respectively, when compared to enrichment culture. The new BD Max Cdiff assay appeared to be an excellent platform for rapid and accurate detection of toxigenic C. difficile.
Details
- Title
- Evaluation of the BD Max Cdiff assay for the detection of toxigenic Clostridium difficile in human stool specimens
- Authors/Creators
- P. Putsathit (Author/Creator) - The University of Western AustraliaJ. Morgan (Author/Creator) - Royal Perth HospitalD. Bradford (Author/Creator) - Pathwest Laboratory MedicineN. Engelhardt (Author/Creator) - Fremantle HospitalT.V. Riley (Author/Creator) - The University of Western Australia
- Publication Details
- Pathology, Vol.47(2), pp.165-168
- Publisher
- Elsevier
- Identifiers
- 991005543218407891
- Copyright
- © 2015 Royal College of Pathologists of Australasia.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.1133 Clostridium Infections
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine