Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers

N. Keane; C. Almeida; S. Roberts; I. Ahmad; S. Mallal; M. John

doi:10.1186/1742-4690-9-S2-P243

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Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers

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Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers

N. Keane, C. Almeida, S. Roberts, I. Ahmad, S. Mallal and M. John

Retrovirology, Vol.9(Suppl 2), P243

2012

DOI: https://doi.org/10.1186/1742-4690-9-S2-P243

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Abstract

The IFNγ ELISpot assay is used widely for high through-put screening of HIV-specific responses in studies of HIV infection and vaccine studies. However, dual production of IFNγ/IL-2 and increased proliferative capacity may be associated with better natural control of HIV infection. We evaluated a novel fluorospot assay enabling the identification of dual IFNγ/IL-2 producing antigen-specific cells and compared it with intracellular cytokine staining by standard flow cytometry in individuals with natural control of HIV-infection.

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Title: Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers
Authors/Creators: N. Keane (Author/Creator) - Murdoch University
C. Almeida (Author/Creator)
S. Roberts (Author/Creator) - Murdoch University
I. Ahmad (Author/Creator) - Murdoch University
S. Mallal (Author/Creator) - Murdoch University
M. John (Author/Creator) - Murdoch University
Publication Details: Retrovirology, Vol.9(Suppl 2), P243
Publisher: BioMed Central
Identifiers: 991005544746307891
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Language: English
Resource Type: Journal article
Note: Poster Presentation at AIDS Vaccine 2012, Boston, 9-12 September, 2012.

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Web Of Science research areas: Virology
ESI research areas: Microbiology