Journal article
Evaluation of the secretory response to endogenous and exogenous prostaglandins in mucosa from the proximal and distal portions of the colon of dogs
American Journal of Veterinary Research, Vol.72(3), pp.404-409
2011
Abstract
Objective - To compare secretory responses to prostaglandin (PG) E 2 in mucosa obtained from the proximal and distal portions of the colon of dogs. Sample - Colonic mucosa from cadavers of 18 clinically normal adult dogs. Procedures - Short-circuit current (Isc) and maximum change in Isc (ΔIsc) in response to administration of 1μM PGE2 were measured across mucosa obtained from the proximal and distal portions of the colon. Responses were evaluated in mucosa (n = 6 dogs) incubated in Ussing chambers with or without 1mM amiloride or without chloride in the Ringer's bathing solution. Responses were also evaluated in mucosa (n = 9 dogs) incubated with or without pretreatment with 1μM indomethacin, with or without amiloride in the subsequent bathing solution. Histologic changes in mucosa from 3 dogs were assessed over time. Results - Isc and ΔIsc were significantly reduced when chloride was removed from, but not when amiloride was added to, the bathing solution and were significantly reduced after pretreatment with indomethacin. The ΔIsc was significantly greater in mucosa from the distal portion of the colon than in the proximal portion of the colon. Histologic changes after incubation for 3 hours were minimal. Conclusions and Clinical Relevance - Isc and ΔIsc resulted from electrogenic chloride secretion. Chloride secretion was reduced when release of PGs was prevented by indomethacin and was induced by administration of PGE2. Chloride secretion in response to PGE2 was greater in mucosa from the distal portion of the colon than in mucosa from the proximal portion of the colon.
Details
- Title
- Evaluation of the secretory response to endogenous and exogenous prostaglandins in mucosa from the proximal and distal portions of the colon of dogs
- Authors/Creators
- M. Omori (Author/Creator)R.C. Hill (Author/Creator)K.C. Scott (Author/Creator)G.D. Lester (Author/Creator)
- Publication Details
- American Journal of Veterinary Research, Vol.72(3), pp.404-409
- Publisher
- American Veterinary Medical Association
- Identifiers
- 991005544480607891
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.195 Neuroendocrine & Intestinal Disorders
- 1.195.1341 Enteric Nervous System
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science