Journal article
Evidence for polynuclear iron (III) clusters in the root nodule bacterium, Rhizobium leguminosarum bv. viciae WSM710
BioMetals, Vol.12(1), pp.73-76
1999
Abstract
Cells of the root nodule bacterium Rhizobium leguminosarum bv. viciae WSM710 were cultured in a medium containing 20 μM 57Fe. Mossbauer spectra of the cells at 5.5 and 3.7 K indicated that the major form of iron present in the cells was in the form of polynuclear iron(III) clusters. At 5.5 K the spectral component associated with these clusters was in the form of a superposition of a broad feature (large magnetic hyperfine field distribution) and a doublet. On lowering the temperature of the cells to 3.7 K, the spectral component was transformed into resolved magnetic hyperfine field splitting which yielded a magnetic hyperfine field of 42.4 T when fitted with broad Lorentzian peaks. These spectral characteristics are typical of the hydrated iron(III) phosphate cores of several bacterioferritins. A small fraction (11%) of the Mossbauer spectral area of the cells was in the form of a doublet which yielded parameters (δ = 1.35 mm/s; ΔE(Q) = 3.15 mm/s) indicative of iron(II). The parameters are very similar to those of a spectral component previously observed in several other microbes and which has been associated with a 2.2 kDa oligomeric iron(II) carbohydrate phosphate.
Details
- Title
- Evidence for polynuclear iron (III) clusters in the root nodule bacterium, Rhizobium leguminosarum bv. viciae WSM710
- Authors/Creators
- T.G. St Pierre (Author/Creator)K.C. Carson (Author/Creator) - Murdoch UniversityJ. Webb (Author/Creator) - Murdoch UniversityA.R. Glenn (Author/Creator) - Murdoch UniversityM.J. Dilworth (Author/Creator) - Murdoch University
- Publication Details
- BioMetals, Vol.12(1), pp.73-76
- Publisher
- Springer Netherlands
- Identifiers
- 991005544261707891
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.839 Outer Membrane
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry