Journal article
Evidence of Altered Guinea Pig Ventricular Cardiomyocyte Protein Expression and Growth in Response to a 5 min in vitro Exposure to H2O2
Journal of Proteome Research, Vol.9(4), pp.1985-1994
2010
Abstract
Oxidative stress and alterations in cellular calcium homeostasis are associated with the development of cardiac hypertrophy. However, the early cellular mechanisms for the development of hypertrophy are not well understood. Guinea pig ventricular myocytes were exposed to 30 μ H2O 2 for 5 min followed by 10 units/mL catalase to degrade the H 2O2, and effects on protein expression were examined 48 h later. Transient exposure to H2O2 increased the level of protein synthesis more than 2-fold, assessed as incorporation of [ 3H]leucine (n = 12; p < 0.05). Cell size was increased slightly, but there was no evidence of major cytoskeletal disorganization assessed using fluorescence microscopy. Changes in the expression of individual proteins were assessed using iTRAQ protein labeling followed by mass spectrometry analysis (LC-MALDI-MSMS); 669 proteins were identified, and transient exposure of myocytes to H2O2 altered expression of 35 proteins that were predominantly mitochondrial in origin, including TCA cycle enzymes and oxidative phosphorylation proteins. Consistent with changes in the expression of mitochondrial proteins, transient exposure of myocytes to H2O 2 increased the magnitude of the mitochondrial NADH signal 10.5 ± 2.3% compared to cells exposed to 0 μH2O2 for 5 min followed by 10 units/mL catalase (n = 8; p < 0.05). In addition, metabolic activity was significantly increased in the myocytes 48 h after transient exposure to H2O2, assessed as formation of formazan from tetrazolium salt. We conclude that a 5 min exposure of ventricular myocytes to 30 μ H2O2 is sufficient to significantly alter protein expression, consistent with the development of hypertrophy in the myocytes. Changes in mitochondrial protein expression and function appear to be early sequelae in the development of hypertrophy.
Details
- Title
- Evidence of Altered Guinea Pig Ventricular Cardiomyocyte Protein Expression and Growth in Response to a 5 min in vitro Exposure to H2O2
- Authors/Creators
- V. Seenarain (Author/Creator) - The University of Western AustraliaH.M. Viola (Author/Creator) - School of Molecular SciencesG. Ravenscroft (Author/Creator) - UWA Centre for Medical ResearchT.M. Casey (Author/Creator) - The University of Western AustraliaR.J. Lipscombe (Author/Creator) - The University of Western AustraliaE. Ingley (Author/Creator) - UWA Centre for Medical ResearchN.G. Laing (Author/Creator) - UWA Centre for Medical ResearchS.D. Bringans (Author/Creator) - The University of Western AustraliaL.C. Hool (Author/Creator) - School of Molecular Sciences
- Publication Details
- Journal of Proteome Research, Vol.9(4), pp.1985-1994
- Publisher
- American Chemical Society
- Identifiers
- 991005540043107891
- Copyright
- © 2010 American Chemical Society.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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