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Journal article
Evolution of novel Mesorhizobium genospecies that competitively and effectively nodulate Cicer arietinum following inoculation with the Australian commercial inoculant strain M. ciceri CC1192
Plant and Soil, Vol.507, pp.397-415
2024
Abstract
Background and aims
Mesorhizobium ciceri CC1192 is the commercial inoculant strain for Cicer arietinum (chickpea) cultivation in Australia, including in the Ord River Irrigation Area (ORIA), where C. arietinum cropping began in 1985. Mesorhizobium strains are known to gain the capacity to nodulate legumes through acquisition of symbiosis Integrative and Conjugative Elements (ICEs), leading to the evolution of novel rhizobia. Here, we assess the impact of symbiosis ICE transfer and compare the genomic diversity and symbiotic effectiveness of C. arietinum nodulating rhizobia from the ORIA.
Methods
Nodule isolates collected from field cultivated C. arietinum were genotyped by RAPD-PCR, and representative strains from each genotype were whole genome sequenced and symbiotically phenotyped in glasshouse conditions to assess N2 fixation effectiveness against CC1192.
Results
A total of 68 nodule isolates, all harbouring the CC1192 symbiosis ICE (ICEMcSym1192), were analysed, with 12 identified as the inoculant strain, and 56 novel strains clustering into ten genotypes. These novel genotypes dominated as nodule occupants across the majority of sites sampled. Nine of the ten representative strains were as effective at N2 fixation as CC1192, with WSM4904 the only ineffective strain. Core genome phylogeny showed the ten strains represent four novel Mesorhizobium genospecies. Novel strains WSM4904 and WSM4906 shared 98.7% sequence identity, yet exhibited very different symbiotic phenotypes.
Conclusions
The CC1192 symbiosis ICE has transferred to a wide diversity of Mesorhizobium spp. in the ORIA. These evolved strains are competitive against CC1192 at nodulating C. arietinum, and the majority are effective symbiotic N2 fixers.
Details
- Title
- Evolution of novel Mesorhizobium genospecies that competitively and effectively nodulate Cicer arietinum following inoculation with the Australian commercial inoculant strain M. ciceri CC1192
- Authors/Creators
- Yvette Hill - Murdoch University, Centre for Sustainable Farming SystemsMacLean Kohlmeier - Murdoch University, Centre for Sustainable Farming SystemsAlireza Agha AmiriGraham O'Hara - Murdoch University, Centre for Sustainable Farming SystemsJason Terpolilli - Murdoch University, Centre for Sustainable Farming Systems
- Publication Details
- Plant and Soil, Vol.507, pp.397-415
- Publisher
- Springer Nature Issued under auspices of the Royal Netherlands Society of Agricultural Science
- Identifiers
- 991005670268307891
- Copyright
- © The Author(s) 2024
- Murdoch Affiliation
- Murdoch University; Centre for Sustainable Farming Systems; School of Medical, Molecular and Forensic Sciences
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Agronomy
- Plant Sciences
- Soil Science
- ESI research areas
- Agricultural Sciences