Journal article
Evolutionary and genomic insights into Clostridioides difficile sequence Type 11: a diverse zoonotic and antimicrobial-resistant lineage of global one health importance
mBio, Vol.10(2), pp.e00446-19
2019
Abstract
Clostridioides difficile (Clostridium difficile) sequence type 11 (ST11) is well established in production animal populations worldwide and contributes considerably to the global burden of C. difficile infection (CDI) in humans. Increasing evidence of shared ancestry and genetic overlap of PCR ribotype 078 (RT078), the most common ST11 sublineage, between human and animal populations suggests that CDI may be a zoonosis. We performed whole-genome sequencing (WGS) on a collection of 207 ST11 and closely related ST258 isolates of human and veterinary/environmental origin, comprising 16 RTs collected from Australia, Asia, Europe, and North America. Core genome single nucleotide variant (SNV) analysis identified multiple intraspecies and interspecies clonal groups (isolates separated by ≤2 core genome SNVs) in all the major RT sublineages: 078, 126, 127, 033, and 288. Clonal groups comprised isolates spread across different states, countries, and continents, indicative of reciprocal long-range dissemination and possible zoonotic/anthroponotic transmission. Antimicrobial resistance genotypes and phenotypes varied across host species, geographic regions, and RTs and included macrolide/lincosamide resistance (Tn6194 [ermB]), tetracycline resistance (Tn6190 [tetM] and Tn6164 [tet44]), and fluoroquinolone resistance (gyrA/B mutations), as well as numerous aminoglycoside resistance cassettes. The population was defined by a large “open” pan-genome (10,378 genes), a remarkably small core genome of 2,058 genes (only 19.8% of the gene pool), and an accessory genome containing a large and diverse collection of important prophages of the Siphoviridae and Myoviridae. This study provides novel insights into strain relatedness and genetic variability of C. difficile ST11, a lineage of global One Health importance.
Details
- Title
- Evolutionary and genomic insights into Clostridioides difficile sequence Type 11: a diverse zoonotic and antimicrobial-resistant lineage of global one health importance
- Authors/Creators
- D.R. Knight (Author/Creator) - Medical, Molecular and Forensic Sciences, Murdoch University, Western Australia, AustraliaB. Kullin (Author/Creator) - University of Cape TownG.O. Androga (Author/Creator) - The University of Western AustraliaF. Barbut (Author/Creator) - Hôpital Saint-AntoineC. Eckert (Author/Creator) - Université Paris CitéS. Johnson (Author/Creator) - Loyola University Medical CenterP. Spigaglia (Author/Creator) - Istituto Superiore di SanitàK. Tateda (Author/Creator) - Toho UniversityP-J Tsai (Author/Creator) - National Cheng Kung UniversityT.V. Riley (Author/Creator) - Medical, Molecular and Forensic Sciences, Murdoch University, Western Australia, AustraliaA.B. Onderdonk (Author/Creator)
- Publication Details
- mBio, Vol.10(2), pp.e00446-19
- Publisher
- American Society for Microbiology
- Identifiers
- 991005543858807891
- Copyright
- © 2019 American Society for Microbiology
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
82 File views/ downloads
58 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.1133 Clostridium Infections
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology