Exacerbation of chronic cigarette-smoke induced lung disease by rhinovirus in mice

A.N. Larcombe; T. Iosifidis; R.E. Foong; L.J. Berry; P.A. Stumbles; D.H. Strickland; P.D. Sly; A. Kicic

doi:10.1016/j.resp.2022.103846

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Exacerbation of chronic cigarette-smoke induced lung disease by rhinovirus in mice

Journal article

Peer reviewed

Exacerbation of chronic cigarette-smoke induced lung disease by rhinovirus in mice

A.N. Larcombe, T. Iosifidis, R.E. Foong, L.J. Berry, P.A. Stumbles, D.H. Strickland, P.D. Sly and A. Kicic

Respiratory Physiology & Neurobiology, Vol.298, Art. 103846

2022

DOI: https://doi.org/10.1016/j.resp.2022.103846

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Abstract

A significant proportion of chronic obstructive pulmonary disease exacerbations are strongly associated with rhinovirus infection (HRV). In this study, we combined long-term cigarette smoke exposure with HRV infection in a mouse model. Our aim was to better understand the effects of HRV infection on such exacerbations, using a realistic method for generating a COPD-like phenotype. After 12-weeks of cigarette smoke exposure, adult female BALB/c mice were infected with HRV-1A and three days later we assessed a range of outcomes including lung volume and function, collected lung tissue for measurement of viral titre, bronchoalveolar lavage for assessment of pulmonary inflammation and levels of key mediators, and fixed lungs for stereological structural analyses. Cigarette smoke exposure alone significantly increased total cells and macrophages, and reduced MIP-2 in bronchoalveolar lavage. HRV-1A infection alone increased neutrophilic inflammation, IP-10 and total protein in lavage and also increased specific airway resistance measured at functional residual capacity. Cigarette smoke and HRV-1A together impacted various lung structural parameters including increasing stereological lung volume. Our results show that long-term cigarette smoke exposure and HRV-1A infection both individually impact respiratory outcomes and combine to alter aspects of lung structure in a mouse model, thus providing insight into the development of future mechanistic studies and appropriate interventions in human disease.

Details

Title: Exacerbation of chronic cigarette-smoke induced lung disease by rhinovirus in mice
Authors/Creators: A.N. Larcombe (Author/Creator) - Curtin University
T. Iosifidis (Author/Creator) - The University of Western Australia
R.E. Foong (Author/Creator) - The Kids Research Institute Australia
L.J. Berry (Author/Creator) - The Kids Research Institute Australia
P.A. Stumbles (Author/Creator) - Murdoch University
D.H. Strickland (Author/Creator) - The Kids Research Institute Australia
P.D. Sly (Author/Creator) - The University of Queensland
A. Kicic (Author/Creator) - The University of Western Australia
Publication Details: Respiratory Physiology & Neurobiology, Vol.298, Art. 103846
Publisher: Elsevier B.V.
Identifiers: 991005544471107891
Copyright: © 2022 Elsevier B.V.
Murdoch Affiliation: College of Science, Health, Engineering and Education
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.65 Allergy; 1.65.192 COPD
Web Of Science research areas: Physiology; Respiratory System
ESI research areas: Clinical Medicine