Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses

C.M. Almeida; S.G. Roberts; R. Laird; E. McKinnon; I. Ahmad; N.M. Keane; A. Chopra; C. Kadie; D. Heckerman; S. Mallal; M. John

doi:10.1016/j.vaccine.2010.06.091

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Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses

Journal article

Open access

Peer reviewed

Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses

C.M. Almeida, S.G. Roberts, R. Laird, E. McKinnon, I. Ahmad, N.M. Keane, A. Chopra, C. Kadie, D. Heckerman, S. Mallal, …

Vaccine, Vol.28(37), pp.6052-6057

2010

DOI: https://doi.org/10.1016/j.vaccine.2010.06.091

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Abstract

Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-γ enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design.

Details

Title: Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses
Authors/Creators: C.M. Almeida (Author/Creator)
S.G. Roberts (Author/Creator) - Murdoch University
R. Laird (Author/Creator) - Murdoch University
E. McKinnon (Author/Creator) - Murdoch University
I. Ahmad (Author/Creator) - Murdoch University
N.M. Keane (Author/Creator) - Murdoch University
A. Chopra (Author/Creator) - Murdoch University
C. Kadie (Author/Creator) - Microsoft (United States)
D. Heckerman (Author/Creator) - Microsoft (Canada)
S. Mallal (Author/Creator) - Murdoch University
M. John (Author/Creator) - Murdoch University
Publication Details: Vaccine, Vol.28(37), pp.6052-6057
Publisher: Elsevier BV
Identifiers: 991005544500007891
Copyright: © 2010 Elsevier Ltd
Murdoch Affiliation: Centre for Clinical Immunology and Biomedical Statistics
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.66 HIV; 1.66.46 HIV Pathogenesis
Web Of Science research areas: Immunology; Medicine, Research & Experimental
ESI research areas: Immunology