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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Exploiting the potential of bioreactors for creating spatial organization in the soil microbiome: A strategy for increasing sustainable agricultural practices
Microorganisms, Vol.10(7), Article 1464
2022
Abstract
Industrial production of synthetic nitrogen fertilizers and their crop application have caused considerable environmental impacts. Some eco-friendly alternatives try to solve them but raise some restrictions. We tested a novel method to produce a nitrogen bioinoculant by enriching a soil microbial community in bioreactors supplying N2 by air pumping. The biomass enriched with diazotrophic bacteria was diluted and applied to N-depleted and sterilized soil of tomato plants. We estimated microbial composition and diversity by 16S rRNA metabarcoding from soil and bioreactors at different run times and during plant uprooting. Bioreactors promoted the N-fixing microbial community and revealed a hided diversity. One hundred twenty-four (124) operational taxonomic units (OTUs) were assigned to bacteria with a greater Shannon diversity during the reactor’s steady state. A total of 753 OTUs were found in the rhizospheres with higher biodiversity when the lowest concentration of bacteria was applied. The apparent bacterial abundance in the batch and continuous bioreactors suggested a more specific functional ecological organization. We demonstrate the usefulness of bioreactors to evidence hidden diversity in the soil when it passes through bioreactors. By obtaining the same growth of inoculated plants and the control with chemical synthesis fertilizers, we evidence the potential of the methodology that we have called directed bioprospecting to grow a complex nitrogen-fixing microbial community. The simplicity of the reactor’s operation makes its application promising for developing countries with low technological progress.
Details
- Title
- Exploiting the potential of bioreactors for creating spatial organization in the soil microbiome: A strategy for increasing sustainable agricultural practices
- Authors/Creators
- C.F. Gutiérrez (Author/Creator) - Universidad del ValleN. Rodríguez-Romero (Author/Creator)S. Egan (Author/Creator)E. Holmes (Author/Creator)J. Sanabria (Author/Creator)
- Publication Details
- Microorganisms, Vol.10(7), Article 1464
- Publisher
- MDPI
- Identifiers
- 991005542309107891
- Copyright
- © 2022 by the authors
- Murdoch Affiliation
- Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.556 Microbial Biocontrol
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology