Expression of the Full-Length and 3′-Spliced cry 1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis

J. Yu; R. Xie; L. Tan; W. Xu; S. Zeng; J. Chen; M. Tang; Y. Pang

doi:10.1007/s00284-001-0092-7

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Expression of the Full-Length and 3′-Spliced cry 1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis

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Expression of the Full-Length and 3′-Spliced cry 1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis

J. Yu, R. Xie, L. Tan, W. Xu, S. Zeng, J. Chen, M. Tang and Y. Pang

Current Microbiology, Vol.45(2), pp.133-138

2002

DOI: https://doi.org/10.1007/s00284-001-0092-7

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Abstract

Bacillus thuringiensis produces a 130 –135-kDa insecticidal protein in the form of bipyramidal crystal which is toxic to lepidopteran larvae. Part of the C-terminal region of the native Cry1Ab was replaced by a heterologous sequence of Cry11Aa C-terminus to get a 3 -spliced cry 1Ab gene. The full-length cry 1Ab and 3 -spliced cry 1Ab, which were both cloned into the E. coli–B. thuringiensis shuttle expression vector pHZB1, were expressed in a 135-kDa crystal protein minus derivative of B. thuringiensis subsp. kyushuensis (4U1-Cry 135 ). The crystal shape of Cry1Ab proteins from both recombinants was regularly bipyramidal, while the crystal size of the intact Cry1Ab was approximately fivefold larger than the 3 -spliced Cry1Ab. In addition, these two kinds of Cry1Ab proteins had similar toxicity against Argyrogramma agnata larvae.

Details

Title: Expression of the Full-Length and 3′-Spliced cry 1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis
Authors/Creators: J. Yu (Author/Creator)
R. Xie (Author/Creator)
L. Tan (Author/Creator)
W. Xu (Author/Creator)
S. Zeng (Author/Creator)
J. Chen (Author/Creator)
M. Tang (Author/Creator)
Y. Pang (Author/Creator)
Publication Details: Current Microbiology, Vol.45(2), pp.133-138
Publisher: Springer Verlag
Identifiers: 991005544569207891
Copyright: © 2002 Springer-Verlag New York Inc.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.32 Entomology; 3.32.827 Insecticide Resistance
Web Of Science research areas: Microbiology
ESI research areas: Microbiology