Journal article
Expression of the noradrenaline transporter and phenylethanolamine N-methyltransferase in normal human adrenal gland and phaeochromocytoma
Cell and Tissue Research, Vol.322(3), pp.443-453
2005
Abstract
Expression of the noradrenaline transporter (NAT) was examined in normal human adrenal medulla and phaeochromocytoma by using immunohistochemistry and confocal microscopy. The enzymes tyrosine hydroxylase (TH) and phenylethanolamine N-methyltransferase (PNMT) were used as catecholamine biosynthetic markers and chromogranin A (CGA) as a marker for secretory granules. Catecholamine content was measured by using high performance liquid chromatography (HPLC). In normal human adrenal medulla (n=5), all chromaffin cells demonstrated strong TH, PNMT and NAT immunoreactivity. NAT was co-localized with PNMT and was located within the cytoplasm with a punctate appearance. Human phaeochromocytomas demonstrated strong TH expression (n=20 samples tested) but variable NAT and PNMT expression (n=24). NAT immunoreactivity ranged from absent (n=3) to weak (n=10) and strong (n=11) and, in some cases, occupied an apparent nuclear location. Unlike the expression seen in normal human adrenal medullary tissue, NAT expression was not consistently co-localized with PNMT. PNMT also showed highly variable expression that was poorly correlated with tumour adrenaline content. Immunoreactivity for CGA was colocalized with NAT within the cytoplasm of normal human chromaffin cells (n=4). This co-localization was not consistent in phaeochromocytoma tumour cells (n=7). The altered pattern of expression for both NAT and PNMT in phaeochromocytoma indicates a significant disruption in the regulation and possibly in the function of these proteins in adrenal medullary tumours.
Details
- Title
- Expression of the noradrenaline transporter and phenylethanolamine N-methyltransferase in normal human adrenal gland and phaeochromocytoma
- Authors/Creators
- S. Cleary (Author/Creator) - Murdoch UniversityF.M. Brouwers (Author/Creator) - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentG. Eisenhofer (Author/Creator) - National Institute of Neurological Disorders and StrokeK. Pacak (Author/Creator) - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentD.L. Christie (Author/Creator)J. Lipski (Author/Creator) - University of AucklandA.R. McNeil (Author/Creator) - Dorevitch PathologyJ.K. Phillips (Author/Creator) - Murdoch University
- Publication Details
- Cell and Tissue Research, Vol.322(3), pp.443-453
- Publisher
- Springer Verlag
- Identifiers
- 991005539989107891
- Copyright
- © Springer-Verlag 2005
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.278 Adrenal Disorders
- 1.278.802 Adrenal Tumors
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Molecular Biology & Genetics