Files and links (1)
CC BY V4.0, Open Access
Journal article
Expression profile of housekeeping genes and Tissue-specific genes in multiple tissues of pigs
Animals (Basel), Vol.12(24), 3539
2022
PMID: 36552460
Abstract
Simple Summary Pig is an important biomedical model and a valuable source of nutrition for humans. The all-sided gene expression profile across multiple tissues engages crucial information to gain insight into their biological functions in pigs. In the present study, a comprehensive analysis has been performed on the gene expression to explore the housekeeping genes, tissue-specific genes and potentially co-expression hub genes across 14 tissues. Our results offer a fresh perspective on the transcriptome regulation of pig tissues. Pigs have become an ideal model system for human disease research and development and an important farm animal that provides a valuable source of nutrition. To profile the all-sided gene expression and their biological functions across multiple tissues, we conducted a comprehensive analysis of gene expression on a large scale around the side of housekeeping genes (HKGs), tissue specific genes (TSGs), and the co-expressed genes in 14 various tissues. In this study, we identified 2351 HKGs and 3018 TSGs across tissues, among which 4 HKGs (COX1, UBB, OAZ1/NPFF) exhibited low variation and high expression levels, and 31 particular TSGs (e.g., PDC, FKBP6, STAT2, and COL1A1) were exclusively expressed in several tissues, including endocrine brain, ovaries, livers, backfat, jejunum, kidneys, lungs, and longissimus dorsi muscles. We also obtained 17 modules with 230 hub genes (HUBGs) by weighted gene co-expression network analysis. On the other hand, HKGs functions were enriched in the signaling pathways of the ribosome, spliceosome, thermogenesis, oxidative phosphorylation, and nucleocytoplasmic transport, which have been highly suggested to involve in the basic biological tissue activities. While TSGs were highly enriched in the signaling pathways that were involved in specific physiological processes, such as the ovarian steroidogenesis pathway in ovaries and the renin-angiotensin system pathway in kidneys. Collectively, these stable, specifical, and co-expressed genes provided useful information for the investigation of the molecular mechanism for an understanding of the genetic and biological processes of complex traits in pigs.
Details
- Title
- Expression profile of housekeeping genes and Tissue-specific genes in multiple tissues of pigs
- Authors/Creators
- Xiangchun Pan - South China Agricultural UniversityJiali Cai - South China Agricultural UniversityYifei Wang - South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou 510642, Peoples R ChinaDantong Xu - South China Agricultural UniversityYao Jiang - Murdoch UniversityWentao Gong - South China Agricultural UniversityYuhan Tian - South China Agricultural UniversityQingpeng Shen - South China Agricultural UniversityZhe Zhang - South China Agricultural UniversityXiaolong Yuan - South China Agricultural UniversityJiaqi Li - South China Agricultural University
- Publication Details
- Animals (Basel), Vol.12(24), 3539
- Publisher
- MDPI
- Number of pages
- 14
- Identifiers
- 991005585961707891
- Copyright
- © 2022 by the authors.
- Murdoch Affiliation
- College of Environmental and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
4 File views/ downloads
39 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1543 Flow Cytometry
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- Veterinary Sciences
- Zoology
- ESI research areas
- Plant & Animal Science